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1991 (1)
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1979 (1)
1Author    Regina Teschner, M. Onika Löffler, Friedhelm SchneiderRequires cookie*
 Title    Lipid Metabolism of Ehrlich Ascites Tumor Cells Grown in Chemically Defined Albumin Medium  
 Abstract    Total lipids o f Ehrlich ascites tumor cells grown in nutrient medium supplemented with 1% bovine serum albumin instead o f 15% native horse serum and rate o f incorporation of [3H] acetate into total lipids are reduced, while the cholesterol content of the cells is higher than of controls grown in normal medium. The lower lipid content o f cells cultured in albumin supplemented medium is explained by a lack o f exogenous lipids, which are normally taken up from the serum containing nutrient medium. A several fold increase of incorporation o f labelled choline and ethanolamine into the phospholipids o f the cells grown in the modified medium is observed. We suggest, that the observed stimulation of the incorporation o f phospholipid precursors reflects changes in the dynamic state o f the membranes produced by serum depletion. 
  Reference    Z. Naturforsch. 35c, 124—128 (1980); received November 5 1979 
  Published    1980 
  Keywords    Cultured Cells, Albumin Containing Medium, Lipid Metabolism 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0124.pdf 
 Identifier    ZNC-1980-35c-0124 
 Volume    35 
2Author    Fumihiko Satoa, YasuyukiY. Am Adaa, Sang Soo, W. Akb, Katsunori Ichinoseb, M. Itsuhiro Kishidab, N. Obutaka Takahashic, Shigeo YoshidabRequires cookie*
 Title    Photoautotrophic Cultured Plant Cells: A Novel System to Survey New Photosynthetic Electron Transport Inhibitors  
 Abstract    The responses o f photoautotrophic (PA) cultured cells o f tobacco (N icotiana tabacum cv. Samsun N N) and liverwort (M archantia polym orpha L.) to thirty-eight cyclohexanedione de­ rivatives were surveyed. Each derivative was also tested for inhibitory activity on photosyn­ thetic electron transport (PET), using isolated thylakoids, and herbicidal activity, using seed­ lings and mature plants. Comparison o f the results from the different assays showed that the responses o f PA cells to each com pound correlated more closely with the responses o f seed­ lings and mature plants than did the results o f the Hill reaction assays. Our findings suggest that PA cultured cells would be a suitable screening material for identifying potential herbi­ cides with PET-inhibiting activity. 
  Reference    Z. Naturforsch. 46c, 563 (1991); received December 12 1990/April 12 1991 
  Published    1991 
  Keywords    Photosynthetic Electron Transport (PET) Herbicides, N ew Screening System, Cultured Cells, Photoautotrophic Cells, Pot Assay 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0563.pdf 
 Identifier    ZNC-1991-46c-0563 
 Volume    46 
3Author    Regina Teschner, Stefan Postius, Monika Löffler, Friedhelm SchneiderRequires cookie*
 Title    Proliferation and Metabolie Activities of Ehrlich Ascites Tumor Cells in Chemically Defined Albumin Media  
 Abstract    , amino acid transport, respiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compar­ ed to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly impaired. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin. 
  Reference    Z. Naturforsch. 34c, 805 (1979); received May 16 1979 
  Published    1979 
  Keywords    Cultured Cells, Chemically Defined Albumin Media, Cell Proliferation Cell proliferation, viability, DNA-, RNA-, protein synthesis 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0805.pdf 
 Identifier    ZNC-1979-34c-0805 
 Volume    34