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1	Author	Regina Teschner, M. Onika Löffler, Friedhelm Schneider	Requires cookie*
	Title	Lipid Metabolism of Ehrlich Ascites Tumor Cells Grown in Chemically Defined Albumin Medium
	Abstract	Total lipids o f Ehrlich ascites tumor cells grown in nutrient medium supplemented with 1% bovine serum albumin instead o f 15% native horse serum and rate o f incorporation of [3H] acetate into total lipids are reduced, while the cholesterol content of the cells is higher than of controls grown in normal medium. The lower lipid content o f cells cultured in albumin supplemented medium is explained by a lack o f exogenous lipids, which are normally taken up from the serum containing nutrient medium. A several fold increase of incorporation o f labelled choline and ethanolamine into the phospholipids o f the cells grown in the modified medium is observed. We suggest, that the observed stimulation of the incorporation o f phospholipid precursors reflects changes in the dynamic state o f the membranes produced by serum depletion.
	Reference	Z. Naturforsch. 35c, 124—128 (1980); received November 5 1979
	Published	1980
	Keywords	Cultured Cells, Albumin Containing Medium, Lipid Metabolism
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0124.pdf
	Identifier	ZNC-1980-35c-0124
	Volume	35

2	Author	Fumihiko Satoa, YasuyukiY. Am Adaa, Sang Soo, W. Akb, Katsunori Ichinoseb, M. Itsuhiro Kishidab, N. Obutaka Takahashic, Shigeo Yoshidab	Requires cookie*
	Title	Photoautotrophic Cultured Plant Cells: A Novel System to Survey New Photosynthetic Electron Transport Inhibitors
	Abstract	The responses o f photoautotrophic (PA) cultured cells o f tobacco (N icotiana tabacum cv. Samsun N N) and liverwort (M archantia polym orpha L.) to thirty-eight cyclohexanedione de rivatives were surveyed. Each derivative was also tested for inhibitory activity on photosyn thetic electron transport (PET), using isolated thylakoids, and herbicidal activity, using seed lings and mature plants. Comparison o f the results from the different assays showed that the responses o f PA cells to each com pound correlated more closely with the responses o f seed lings and mature plants than did the results o f the Hill reaction assays. Our findings suggest that PA cultured cells would be a suitable screening material for identifying potential herbi cides with PET-inhibiting activity.
	Reference	Z. Naturforsch. 46c, 563 (1991); received December 12 1990/April 12 1991
	Published	1991
	Keywords	Photosynthetic Electron Transport (PET) Herbicides, N ew Screening System, Cultured Cells, Photoautotrophic Cells, Pot Assay
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	TEI-XML for	default:Reihe_C/46/ZNC-1991-46c-0563.pdf
	Identifier	ZNC-1991-46c-0563
	Volume	46

3	Author	Regina Teschner, Stefan Postius, Monika Löffler, Friedhelm Schneider	Requires cookie*
	Title	Proliferation and Metabolie Activities of Ehrlich Ascites Tumor Cells in Chemically Defined Albumin Media
	Abstract	, amino acid transport, respiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compar ed to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly impaired. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin.
	Reference	Z. Naturforsch. 34c, 805 (1979); received May 16 1979
	Published	1979
	Keywords	Cultured Cells, Chemically Defined Albumin Media, Cell Proliferation Cell proliferation, viability, DNA-, RNA-, protein synthesis
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	TEI-XML for	default:Reihe_C/34/ZNC-1979-34c-0805.pdf
	Identifier	ZNC-1979-34c-0805
	Volume	34