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1978 (1)
1Author    W. Olfram Koller, Jürgen Frevert, H. Elm, Ut KindiRequires cookie*
 Title    Incomplete Glyoxysomes Appearing at a Late Stage of Maturation of Cucumber Seeds  
 Abstract    Seeds of cucumber fruits at a late stage o f ripening were analyzed for microbodies and micro­ body components. On isopycnic density gradient centrifugation of homogenates in the presence o f EDTA, several particulate fractions were obtained: a light membraneous fraction (density d = 1.09-1.11 kg x I-1), a mitochondria-enriched fraction (d = 1.21 kg x I-1), a microbody-enrich­ ed fraction (d = 1.23 kg x I-1), and a protein body fraction (d = 1.26-1.29 kg x I-1). Microbo­ dies were revealed by exactly coinciding peaks o f malate synthase, catalase and crotonase; small proportions of citrate synthase and malate dehydrogenase were also present in this zone. Isocitrate lyase activity, however, did not occur in the seeds at this stage. The examination of enzyme activi­ ties indicated the presence o f microbodies which cannot function as competent glyoxysomes be­ cause of the lack o f isocitrate lyase. Moreover, de novo synthesis from [3H] leucine could be de­ monstrated for malate synthase by means of immunoprecipitation of newly synthesized malate synthase and subsequent electrophoretic analysis. 
  Reference    Z. Naturforsch. 34c, 1232 (1979); received July 23 1979 
  Published    1979 
  Keywords    Microbodies, Malate Synthase, Cucumis sativus, Seed Ripening 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1232.pdf 
 Identifier    ZNC-1979-34c-1232 
 Volume    34 
2Author    W. Olfram Koller, Helm Ut KindlRequires cookie*
 Title    Rates of de novo Synthesis of Malate Synthase and Albumins during the Very Early Phase of Germination  
 Abstract    Malate synthase is synthesized de novo in the very early phase of germination. Its molecular and immunological properties do not differ from those o f malate synthase from fully developed cotyl­ edons. Radioactive leucine was administered to dry seeds of cucumber, and its incorporation into proteins o f cotyledons was examined after 2 days o f germination. The specific radioactivity of ma­ late synthase, purified by immunoprecipitation and electrophoresis on polyacrylamide gel, was only 1/20 the average value of the total albumin fraction. The minimal incorporation documented by the comparatively low specific activity o f isolated malate synthase is discussed in relation to the large pool of malate synthase already present in dry seeds. 
  Reference    Z. Naturforsch. 34c, 1237 (1979); received July 23 1979 
  Published    1979 
  Keywords    Protein Synthesis, Albumins, Malate Synthase, Cucumis sativus 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1237.pdf 
 Identifier    ZNC-1979-34c-1237 
 Volume    34 
3Author    W. Olfram Koller, Helmut KindiRequires cookie*
 Title    Studies Supporting the Concept of Glyoxyperoxisomes as Intermediary Organelles in Transformation of Glyoxysomes into Peroxisomes  
 Abstract    Interm ediary forms of microbodies, glyoxyperoxisomes, are most likely synthesized at the stage of replacement of glyoxysomes by peroxisomes during the greening of fatstoring cells of cucumber cotyledons. This hypothesis is supported by three lines of results: 1. The levels of both glycollate oxidase and malate synthase are increased in the m icrobody fractions upon illum ination of cotyle­ dons. 2. Both peroxisomal and glyoxysomal marker enzymes are localized in organelles which exhibit the same buoyant densities. 3. De novo synthesis of m alate synthase takes place even when irradiation already leads to a drastic increase of glycollate oxidase. T h e amount of m alate synthase synthesized is almost identical in the light-induced system and in the dark control. 
  Reference    Z. Naturforsch. 33c, 962 (1978); received July 24 1978 
  Published    1978 
  Keywords    Enzyme Synthesis, M alate Synthase, Glycollate Oxidase, Glyoxyperoxisomes, Cucumis sativus 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0962.pdf 
 Identifier    ZNC-1978-33c-0962 
 Volume    33 
4Author    Dirk Redecker, InkeS. Feder, Pablo Vinuesa, Thomas Batinic, Ulrike Schulz, Kerstin Kosch, Dietrich WernerRequires cookie*
 Title    Genotypic Characterization by DNA Fingerprinting  
 Abstract    A DNA probe specific for biocontrol strains of Pseudomonas was produced by screening randomly amplified polymorphic DNA (R A P D) PCR fragments. Specificity of the probe was assessed by dot blot and colony hybridization. It was used to specifically determine the population of these strains on roots of Cucumis sativus cv. Delikatess. Two polymorphic R A PD fragments of 750 bp, and 550 bp showed identical specificity. The biocontrol strain Pseudomonas sp. W34 was shown to be competitive in the rhizosphere of cucumber and to maintain a stable population for at least 10 days when inoculated on the seed. The phylogenetic relationships between the biocontrol and reference strains were analyzed at the strain level by means of R A PD and repetitive sequence-based PCR genomic finger­ printing (rep-PCR), and at higher taxonomic levels by means of amplified 16S ribosomal DNA restriction analysis A R D R A . It was shown that the antagonistic strains are closely related, forming a separate cluster from other non-antagonistic and reference Pseudomonas strains, their taxonomic placement remaining uncertain. 
  Reference    Z. Naturforsch. 54c, 359 (1999); received December 10 1998/January 27 1999 
  Published    1999 
  Keywords    Pseudomonas sp W34, Biocontrol, Cucumis sativus, DNA Fingerprinting, DNA Probe 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0359.pdf 
 Identifier    ZNC-1999-54c-0359 
 Volume    54 
5Author    ThiloC. Fischer, Sabine Groner, Ulrike Zentgraf, Vera HemlebenRequires cookie*
 Title    Evidence for Nucleosomal Phasing and a Novel Protein Specifically Binding to Cucumber Satellite DNA  
 Abstract    The nucleosomal organization and the protein-binding capability of highly repeated and methylated satellite DNA of cucumber (Cucumis sativus L.), comprising approx. 30% of the genome, were analyzed. Nucleosomal core DNA from satellite type I was prepared after micrococcal nuclease digestion of chromatin and sequenced. Most of the core sequences ob­ tained could be grouped in two main (A and B) and two minor groups (C and D) indicating a specific and complex phasing of nucleosomes on this satellite DNA. In vitro, gel retarda­ tion assays with cloned satellite DNA repeats (types I-IV) demonstrated a specific binding of nuclear proteins. These specific binding effects are also obtained with genomic, in vivo methylated and sequence heterogeneous (1 to 10% diversity) satellite type I DNA. For the first time in plants, a satellite DNA-binding protein with an apparent molecular weight of 14 kDa (SAT 14) was identified. 
  Reference    Z. Naturforsch. 49c, 79—86 (1994); received October 28/November 121993 
  Published    1994 
  Keywords    Cucumis sativus, DNA Methylation, DNA-Protein Interaction, Nucleosomal Phasing, Satellite DNA 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0079.pdf 
 Identifier    ZNC-1994-49c-0079 
 Volume    49