| | 1 | Author
| SatishK. Sharma, JinnieM. Garrett, StewartA. Brown | Requires cookie* | | | Title
| Separation of the S-Adenosylmethionine: 5-and 8-Hydroxyfuranocoumarin O-Methyltransferases of Ruta graveolens L. by General Ligand Affinity Chromatography  | | | | Abstract
| Two S-adenosyl-L-methionine:furanocoumarin O-methyltransferases of R . graveolens, acting at the 5-and 8-hydroxyls of the psoralen nucleus, were completely resolved by adsorption on a general affinity ligand, 5 -(3-carboxypropanamido) xanthotoxin, followed by specific desorption by bergaptol and xanthotoxol, respectively. The 5-O-methyltransferase was purified 450-fold by this procedure, the 8-O-methyltransferase 112-fold, and both enzyme fractions were electrophoretically homogeneous. No resolution could be achieved of the activity against two 5-hydroxypsoralens or of the activity against two 8-hydroxypsoralens, and conclusive evidence is presented for the existence of only one 5-O-methyltransferase and only one 8-O-methyltransferase acting on linear furanocoumarins. In tro d u c tio n | | | |
Reference
| Z. Naturforsch. 34c, 387 (1979); received March 9 1979 | | | |
Published
| 1979 | | | |
Keywords
| Coumarins, Furanocoumarins, O-Methyltransferases, Affinity Chromatography, R u ta graveolens | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0387.pdf | | | | Identifier
| ZNC-1979-34c-0387 | | | | Volume
| 34 | |
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