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1980 (1)
1Author    Günter Pilwat, Peter Washausen, Joachim Klein, Ulrich ZimmermannRequires cookie*
 Title    Immobilization of Human Red Blood Cells  
 Abstract    H uman red blood cells were immobilized in an alginate network which was cross-linked with C a2+ ions. The im ­ mobilized cells were stored for longer than 5 weeks at 4 °C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The im ­ mobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the im mobilized and released cells retain their biconcave shape over the storage period. Measurements of the released cell popula­ tion in a hydrodynamically focussing Coulter C ounter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity have not changed in contrast to control m easurem ents on red blood cells stored conventionally in suspension indicating that im mobilization preserves cellular functions. 
  Reference    Z. Naturforsch. 35c, 352—356 (1980); received December 4 1979 
  Published    1980 
  Keywords    Red Blood Cells, Immobilization, Reversible Gels, Electri­ cal Breakdown, Coulter Counter 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0352_n.pdf 
 Identifier    ZNC-1980-35c-0352_n 
 Volume    35