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1982 (1)
1981 (1)
1978 (1)
1Author    K. L. Friedm, R. EgelRequires cookie*
 Title    Protein Patterns during Sporulation in Fission Yeast  
 Abstract    Various strains (wild-type and m utants) of Schizosaccharom yces pom be were subjected to sporulation conditions, and analysed with regard to changes in their protein patterns. Trisbuffer-soluble proteins (TS) and DNA-binding proteins (DB) were denatured and separated by poly­ acrylam ide electrophoresis. A fter endogenous nitrogen depletion (the apparent trigger of conjuga­ tion and sporulation) the following alterations were observed: larger TS-proteins were proteolytical-ly degraded; the pattern of DB-proteins underwent several selective changes (reduction, enforce­ ment, or new appearance of individual bands) ; an endonucleolytic DNase activity appeared. Most of these changes were expressed before conjugation, and did not appear in sterile strains. They could, however, be provoked by an abrupt shift to nitrogen-free medium, even in sterile strains. Inhibition studies with cycloheximide revealed at least two phases of protein synthesis specifically needed for sporulation: a very sensitive phase before conjugation, and a more resistant phase lasting until 0.5 h before spore form ation. Medium exchange between heterothallic cultures (h+ and h~) effected certain sporulation-specific alterations of the DB-protein pattern, indicative of diffusible mating-type factors in the culture fluid. 
  Reference    Z. Naturforsch. 33c, 84 (1978); received December 14 1977 
  Published    1978 
  Keywords    Schizosaccharom yces pom be, Conjugation, Sporulation, Endonuclease, Gel Electrophoresis 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0084.pdf 
 Identifier    ZNC-1978-33c-0084 
 Volume    33 
2Author    Michael Entzeroth, Lothar JaenickeRequires cookie*
 Title    Blepharisma japonicum Synthesis of Blepharismone, the Low-Molecular Conjugation Hormone of Blepharisma japonicum  
 Abstract    An effective and versatile synthesis of 5-hydroxytrypto-phane derivatives offers good access to the conjugation in­ ducing hormone blepharismone o f the ciliate Blepharisma japonicum and to derivatives and analogues of this biolog­ ically active compound. 5'-Fluoroblepharismone and 2'-des-formamidoblepharismone induce conjugation at 500 and 1200 times, respectively, the concentration of the true effector. At high concentration, however, the 2'-des-form-amido derivative desensitizes the receptor system for the natural gamone. 
  Reference    Z. Naturforsch. 36c, 180 (1981); eingegangen am 20. Oktober 1980 
  Published    1981 
  Keywords    Blepharisma japonicum, Blepharismone, Conjugation, Gamones, Hormones 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0180_n.pdf 
 Identifier    ZNC-1981-36c-0180_n 
 Volume    36 
3Author    Michael Entzeroth, Lothar JaenickeRequires cookie*
 Title    von Blepharisma japonicum Structure-Activity-Relation of Analogues and Homologues of Blepharismone, the Low-M olecular Conjugation Hormone of Blepharisma japonicum  
 Abstract    Some functional derivatives o f blepharismone, the low-m olecular conjugation hormone o f Blepharisma japonicum, were synthesised and tested for their biological activity. The lipophilicity constant o f the substituent in 5-position o f the aromatic system directly correlates with the conjugation-inducing effect o f this substance. Any change in the N -form yl-position leads to com pounds less active than blepharismone, non-active or even inhibitory. Blepharismone-related kynurenine derivatives are inhibitors o f the gamone activity depending on structural relationship. 
  Reference    Z. Naturforsch. 37c, 1136—1140 (1982); received August 2/Septem ber 20 1982 
  Published    1982 
  Keywords    Blepharisma japonicu m, Blepharismone, Conjugation, Hormones, Receptors 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-1136.pdf 
 Identifier    ZNC-1982-37c-1136 
 Volume    37