ZfN: Search Results

1	Author	K. L. Friedm, R. Egel	Requires cookie*
	Title	Protein Patterns during Sporulation in Fission Yeast
	Abstract	Various strains (wild-type and m utants) of Schizosaccharom yces pom be were subjected to sporulation conditions, and analysed with regard to changes in their protein patterns. Trisbuffer-soluble proteins (TS) and DNA-binding proteins (DB) were denatured and separated by poly acrylam ide electrophoresis. A fter endogenous nitrogen depletion (the apparent trigger of conjuga tion and sporulation) the following alterations were observed: larger TS-proteins were proteolytical-ly degraded; the pattern of DB-proteins underwent several selective changes (reduction, enforce ment, or new appearance of individual bands) ; an endonucleolytic DNase activity appeared. Most of these changes were expressed before conjugation, and did not appear in sterile strains. They could, however, be provoked by an abrupt shift to nitrogen-free medium, even in sterile strains. Inhibition studies with cycloheximide revealed at least two phases of protein synthesis specifically needed for sporulation: a very sensitive phase before conjugation, and a more resistant phase lasting until 0.5 h before spore form ation. Medium exchange between heterothallic cultures (h+ and h~) effected certain sporulation-specific alterations of the DB-protein pattern, indicative of diffusible mating-type factors in the culture fluid.
	Reference	Z. Naturforsch. 33c, 84 (1978); received December 14 1977
	Published	1978
	Keywords	Schizosaccharom yces pom be, Conjugation, Sporulation, Endonuclease, Gel Electrophoresis
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	TEI-XML for	default:Reihe_C/33/ZNC-1978-33c-0084.pdf
	Identifier	ZNC-1978-33c-0084
	Volume	33

2	Author	Michael Entzeroth, Lothar Jaenicke	Requires cookie*
	Title	Blepharisma japonicum Synthesis of Blepharismone, the Low-Molecular Conjugation Hormone of Blepharisma japonicum
	Abstract	An effective and versatile synthesis of 5-hydroxytrypto-phane derivatives offers good access to the conjugation in ducing hormone blepharismone o f the ciliate Blepharisma japonicum and to derivatives and analogues of this biolog ically active compound. 5'-Fluoroblepharismone and 2'-des-formamidoblepharismone induce conjugation at 500 and 1200 times, respectively, the concentration of the true effector. At high concentration, however, the 2'-des-form-amido derivative desensitizes the receptor system for the natural gamone.
	Reference	Z. Naturforsch. 36c, 180 (1981); eingegangen am 20. Oktober 1980
	Published	1981
	Keywords	Blepharisma japonicum, Blepharismone, Conjugation, Gamones, Hormones
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	TEI-XML for	default:Reihe_C/36/ZNC-1981-36c-0180_n.pdf
	Identifier	ZNC-1981-36c-0180_n
	Volume	36

3	Author	Michael Entzeroth, Lothar Jaenicke	Requires cookie*
	Title	von Blepharisma japonicum Structure-Activity-Relation of Analogues and Homologues of Blepharismone, the Low-M olecular Conjugation Hormone of Blepharisma japonicum
	Abstract	Some functional derivatives o f blepharismone, the low-m olecular conjugation hormone o f Blepharisma japonicum, were synthesised and tested for their biological activity. The lipophilicity constant o f the substituent in 5-position o f the aromatic system directly correlates with the conjugation-inducing effect o f this substance. Any change in the N -form yl-position leads to com pounds less active than blepharismone, non-active or even inhibitory. Blepharismone-related kynurenine derivatives are inhibitors o f the gamone activity depending on structural relationship.
	Reference	Z. Naturforsch. 37c, 1136—1140 (1982); received August 2/Septem ber 20 1982
	Published	1982
	Keywords	Blepharisma japonicu m, Blepharismone, Conjugation, Hormones, Receptors
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	TEI-XML for	default:Reihe_C/37/ZNC-1982-37c-1136.pdf
	Identifier	ZNC-1982-37c-1136
	Volume	37