| | 1 | Author
| M. Otoo Sumidaa, Shinjiro Niwata3, Takaharu Tanaka3, Tadahide Furunob, M. Amoru, Nakanishib, Ko Wakabayashic, Peter Bögerd | Requires cookie* | | | Title
| Accumulation of Protoporphyrinogen IX Induced by Acifluorfen Methyl  | | | | Abstract
| Confocal fluorescence microscopic images were used to investigate the accumulation site of protoporphyrin IX (PPIX) within liverwort cells (Marchantia polymorpha L.) treated with peroxidizing herbicides as acifluorfen methyl (AFM). A high level of PPIX accumulation was observed in the cells during 12 h to 24 h after the addition of AFM . The results obtained from confocal fluorescence microscopic images gave clear evidence that the accumulation of PPIX occurred only in the chloroplasts, but was not observed in the cytosol or at the plasma membrane. The presence of PPIX in the chloroplasts strongly suggests that protoporphyrino gen (Protogen) accumulates by inhibition of protoporphyrinogen oxidase (Protox) which is the target enzyme for peroxidizing herbicides. The plastidic occurrence of PPIX provides evidence of either the presence of an additional herbicide-resistant Protox or of a non-enzy-matic Protogen-oxidation system in the Marchantia chloroplast. | | | |
Reference
| Z. Naturforsch. 51c, 174 (1996); received D ecem ber 8/Decem ber 20 1995 | | | |
Published
| 1996 | | | |
Keywords
| Protoporphyrinogen, Protoporphyrinogen Localization, Protoporphyrinogen Oxidation, Liverwort Cell Culture, Confocal Fluorescence Microscopy | | | |
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| default:Reihe_C/51/ZNC-1996-51c-0174.pdf | | | | Identifier
| ZNC-1996-51c-0174 | | | | Volume
| 51 | |
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