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1975 (1)
1Author    Akira TaketoRequires cookie*
 Title    Ba2+-Induced Competence for Transfecting D NA  
 Abstract    Effect of alkaline earth metal ions on induction of the competence for DNA transfection was investigated. Unlike spheroplasts, the bulk of the bacteria treated with these ions retains colony-forming ability. The order of effectiveness for transfection of replicative-form DNA has been found to be Ba2+> C a 2+> S r 2+> M g 2+. The competence of Ba2+-treated cells is 3 to 5 times higher than that of Ca^-treated bacteria and about 40 times higher than that of lysozyme-EDTA sphero­ plasts. The Ba^-dependent transfection is cryophilic and formation of the infective complex occurs very rapidly at 0 °C, but not at 37 °C. 
  Reference    (Z. Naturforsch. 30c, 520—522 [1975]; received March 11 1975) 
  Published    1975 
  Keywords    Ba2+, Competence, DNA, Escherichia coli, Transfection 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0520.pdf 
 Identifier    ZNC-1975-30c-0520 
 Volume    30