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'Circadian Rhythm' in keywords Facet   Publication Year 1998  [X]
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1998[X]
1Author    A. Kiinnea, E. Pistoriusb, K. Kloppstechc, E. De, G. RootdRequires cookie*
 Title    Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control  
 Abstract    Two proteins with apparent molecular masses of 17 and 24 kD that are synthesized in a circadian manner in the phytoflagellate Euglena gracilis, were recognized as proteins belong­ ing to the family of light-harvesting-chlorophyll-proteins (LHCPs) of class 1(17 kD) and of class II (24 kD). Identification was achieved by N-terminal sequencing of the proteins iso­ lated from two-dimensional polyacrylamide gels and by detection with an anti-LHCP II se­ rum. While it was found that the total am ount of LHCPs remains almost constant, when Euglena is grown under diurnal conditions (12 h light and 12 h dark), we could show that the amount of newly synthesized 17 and 24 kD proteins varies about 20-fold with a maximum of synthesis in the light phase. In contrast, the analysis of the mRNA levels at different times revealed only minor differences in the stationary concentration of the LHCP specific mRNA, indicating that the control of LHCP synthesis is at the translational level. Principally, the same finding was obtained using inhibitors of transcription. Thus, it is concluded that the expression of LHCPs in Euglena gracilis in contrast to that of higher plants is primarily regulated at the translational level. 
  Reference    Z. Naturforsch. 53c, 1017—1026 (1998); received July 7/August 3 1998 
  Published    1998 
  Keywords    Euglena gracilis, Circadian Rhythm, Translational Control, Protein Synthesis, Light-Harvesting-Chlorophyll-Proteins 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-1017.pdf 
 Identifier    ZNC-1998-53c-1017 
 Volume    53