| 1 | Author | Peter Gräber, Sathamm Saphon | Requires cookie* |
| Title | Conformational Changes of the Membrane-Bound ATPase of Bacterial Chromatophores Revealed by Fluorescence Changes of Fluorescamine-Labelled Coupling Factors  | ||
| Abstract | The solubilized coupling factor (Ft) of Rps. sphaeroides chromatophores was allowed to react with fiuorescamine which led to a fluorescence labelled F t . After reconstitution with the depleted membranes the fluorescence-labelled Fj was shown to restore photophosphorylation in continuous light in a similar way to the non-labelled F t . In parallel, a decrease of the fluorescence emission of the labelled and reconstituted coupling factor was observed. The solubilized and labelled F x showed also a fluorescence decrease as the polarity of the medium was increased. In single turnover flashes the fluorescence change was found to be inhibited by an uncoupling agent such as FCCP. The kinetics of the change were sensitive to phosphorylating agents and to an "energy transfer inhibitor" such as venturicidin. It is suggested that the observed fluorescence changes reflect conformational changes of the ATPase enzyme complex. | ||
| Reference | Z. Naturforsch. 33c, 421—427 (1978); received March 28 1978 | ||
| Published | 1978 | ||
| Keywords | Chromatophores Coupling Factors, Fluorescence-Labelling, Conformational Changes | ||
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