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1Author    Heinz Faulstich, Dionysos Georgopoulos, Maria Bloching, Theodor WielandRequires cookie*
 Title    Analysis of the Toxins of Amanitin-Containing Mushrooms  
 Abstract    We have recently developed a method for the analysis of toxins in single specimens of the mush­ room Amanita phalloides (Fr.) Seer.1. Separation of the different toxins was achieved by column chro­ matography, on Sephadex LH20 in water, of a methanolic extract of fresh or dried mushrooms, followed by further purification on thinlayers of silica gel, spectrophotometric evaluation, and, final­ ly, identification of the toxins by amino acid analy­ ses of their y-hydroxylated leucines or isoleucines as lactones. This procedure afforded the quantitative determination of up to 10 toxins in 2 samples of A. phalloides from different sources. 
  Reference    (Z. Naturforsch. 29c, 86 [1974]; received October 25 1973) 
  Published    1974 
  Keywords    Amanitins, Phallotoxins, Toxic Mushrooms, Chromatography 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0086_n.pdf 
 Identifier    ZNC-1974-29c-0086_n 
 Volume    29 
2Author    Cornelius LützRequires cookie*
 Title    The Determ ination o f Prolam ellar Bodies and Saponins in E tioplasts and Leaves o f A vena sativa L  
 Abstract    Prolamellar body tubules in etioplasts are composed mainly of two saponins. The determination o f these sapo-nins in leaves, etioplasts or subfractions of etioplasts is re­ commended to evaluate the concentration of these tubules in a given fraction. Using a densitometer after TLC-separa-tion of the saponins measurements are carried out in a short time with high accurancy. Also the absolute concen­ tration of these saponins can be determined. Problems in the quantification o f the saponins in leaf samples can be overcame with filters of different wavelengths. 
  Reference    Z. Naturforsch. 35c, 519—521 (1980); received January 21 1980 
  Published    1980 
  Keywords    Etioplasts, Prolamellar Bodies, Saponins, Chromatography 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0519_n.pdf 
 Identifier    ZNC-1980-35c-0519_n 
 Volume    35 
3Author    C. Köster, J. Grotemeyer, E. W. SchlagRequires cookie*
 Title    A High Pressure Pulsed Expansion Valve for Gases, Liquids, and Supercritical Fluids  
 Abstract    A novel design of a pulsed valve for coupling chromatographic techniques with gaseous and liquid mobile phases to a time-of-flight mass spectrometer with multiphoton ionization (MUPI) is pre-sented. The valve can be operated in low pressure regions (<10 bar) up to temperatures of 350 °C and at higher pressures (300 bar) up to temperatures of 200 °C. Pulse widths lower than 100 |is could be measured. First results demonstrate the ability of interfacing of liquid chromatography to MUPI-mass spectrometry. Additional coupling of C0 2 -laser desorption to the valve allows the interface to be used for mass spectrometric measurements of nonvolatile biomolecules. 
  Reference    Z. Naturforsch. 45a, 1285—1292 (1990); received October 18 1990 
  Published    1990 
  Keywords    High pressure valve, Chromatography, Multiphoton-ionization mass spectrometry 
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 TEI-XML for    default:Reihe_A/45/ZNA-1990-45a-1285.pdf 
 Identifier    ZNA-1990-45a-1285 
 Volume    45 
4Author    Thorsten Kemmer, Hans Brockmann, Nikolaus RischRequires cookie*
 Title    Die Einzelkomponenten der Bacteriophäophorbidmethylester von Bacteriochlorophyll c und d The Individual Components of Bacteriophaeophorbide Methyl Esters from Bacteriochlorophyll c and d  
 Abstract    The preparative column chromatographic separation on small grained silica gel of the bacteriophaeophorbide-c-and -d-methyl esters into their homologous and isomeric components is described. The influence of particular regions of the molecule, which carry different alkyl-substituents, on the chromatographic separability is selectively enhanced through the variation of the eluent. Mass and ifl NMR spectra of the pure components are discussed. Einführung Von den in Chlorobiaceae vorkommenden Bacterio-chlorophyllen c und d ist schon seit den Untersuchun-gen von Holt und Mitarbeitern [1, 2] bekannt, daß sie in der Natur als komplexe Gemische isomerer und homologer Verbindungen vorkommen. Eine Trennung in die einzelnen Komponenten ist für die aus den Chlorophyllen durch Hydrolyse leicht zu-gänglichen Phäophorbide durch Verteilen zwischen Ether und Salzsäure an Celite-Säulen beschrieben worden [3-5]. Wieweit dabei die einzelnen Kompo-CH3 nenten wirklich in reiner Form erhalten wurden, ist heute schwer zu überprüfen; bei den sich anschlie-ßenden physikalischen und chemischen Untersu-chungen wurden nämlich zum Teil widersprüchliche Resultate erhalten [6, 7]. Dennoch konnten Holt und Mitarbeiter [1, 2] aus ihren Untersuchungs-ergebnissen die Konstitutionsformeln 1 und 2 für die Bacteriochlorophylle c und d ableiten, die trotz zeitweiliger Kritik [7-9] durch neuere Arbeiten nur bestätigt wurden [10, 11]. Chlorobium-Bacteriochlorophylle cd) und d(2) 
  Reference    Z. Naturforsch. 34b, 633—637 (1979); eingegangen am 10. August 1978 
  Published    1979 
  Keywords    Chromatography, Separation, Bacteriochlorophylls, X H NMR 
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 TEI-XML for    default:Reihe_B/34/ZNB-1979-34b-0633.pdf 
 Identifier    ZNB-1979-34b-0633 
 Volume    34 
5Author    H. Artm, Ut KayserRequires cookie*
 Title    The Use of Argentation Chromatography for the Analysis of Fatty Acid Esters of Polyenes: The Structure of Carotenoid Esters of A glais urticae (Lepidoptera, Insecta)  
 Abstract    Argentation Thin-layer systems for argentation chromatography of fatty-acid esters of carotenoids have been developed. As two-dimensional reversed-phase partition system on paraffine impregnated cellulose this method permits a clear discrimination between saturated and unsaturated fatty acids. By adsorption on silver nitrate containing silica gel-G separation of carotenoid esters according to the degree of unsaturation of their fatty acids was established. With the use of known esters for comparison the fatty acids of whole carotenoid esters can be successfully analysed from minute amounts. Using these methods the carotenoid esters of pupae of A glais u rticae have been studied. The pupae contain 5.3% /?-carotene, 46% lutein diester, 7.8% lutein 3-monoester, 11.7% lutein 3'-mono-ester, and 29.2% unesterified lutein. The fatty acids of the esters are linoleic acid (18:2) and linolenic acid (18:3) only. The diester fraction was composed of 70% dilinolenate, 25% linolenate-linoleate, and 5% dilinoleate. The combined monoesters consisted of 81% linolenate and 19% linoleate. The two main diesters could be isolated in a preparative scale and their structure verified by mass spectrometry. On the whole, in A glais pupae 6.2 fig linolenic acid and 1.4 [xg linoleic acid are bound to lutein. Since polyunsaturated fatty acids are of dietary origin, and represent essential factors for insect development, it is concluded, that their esterification with carotenoids may be a mode of storage comparable to the formation of glycerides. 
  Reference    (Z. Naturforsch. 30c, 369—378 [1975]; received February 19 1975) 
  Published    1975 
  Keywords    Chromatography, Fatty Acids, Carotenoid Esters, Insect Lipids 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0369.pdf 
 Identifier    ZNC-1975-30c-0369 
 Volume    30 
6Author    Ruth Seeger, Tjakko StijveRequires cookie*
 Title    Amanitin Content and Toxicity of Amanita verna Bull  
 Abstract    The < x-, /?-, and ^-amanitin content of 11 samples of Amanita verna Bull., collected during 1975 — 1978 in Germany and Switzerland, has been determined by high performance thin-layer chromatography (HPTLC) of crude methanolic extracts. The toxicity (i. v. LD50 for mice of defatted, lyophilized, methanolic extracts) of 3 samples has been compared with that of A. phal­ loides from the same site of collection. The amanitin content of A. verna ranged from 2250 to 4570 mg/kg dry weight; the fungi con­ tained almost as much ß-as a-amanitin, whereas the y-amanitin content amounted to about 12% of the total amanitin. A. verna contained less amanitin (65% on the average) than A. phalloides from the same collection site, but it was not significantly less toxic, since the phallotoxins contributed to the toxicity of either species in our tests. 
  Reference    Z. Naturforsch. 34c, 330 (1979); received February 5 1979 
  Published    1979 
  Keywords    Amanitins, Amanita verna, Amanita phalloides, Mushroom Poisoning, Chromatography 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0330.pdf 
 Identifier    ZNC-1979-34c-0330 
 Volume    34