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1Author    Peter Flesch, Lutz RobbelRequires cookie*
 Title    Über die Bildung von Cholesterin durch Botrytis cinerea nach Lanosterinzugabe The Production of Cholesterol by Botrytis cinerea after Addition of Lanosterol  
 Abstract    The fungus Botrytis cinerea, which is found on the grapes, is able to produce cholesterol after addition of lanosterol to the culture medium. The identification o f cholesterol is carried out with different analytical methods including mass spectrometry. U nder the same conditions ergosterol arises from squalene and not cholesterol. 
  Reference    Z. Naturforsch. 35c, 88—9 (1980); eingegangen am 16. 
  Published    1980 
  Keywords    Mycosterols, Lanosterol, Cholesterol, Botrytis cinerea 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0088.pdf 
 Identifier    ZNC-1980-35c-0088 
 Volume    35 
2Author    N. Gulfo, R. Bartucci, L. SportelliRequires cookie*
 Title    Effect of Inhalation Anesthetics on Spin-Labeled Cholesterol Containing DPPC Vesicles  
 Abstract    We have investigated by means of electron spin resonance (ESR) spectroscopy the influence of three inhalation anesthetics, i.e. halothane, chloroform and diethyl ether, on the interfacial and hydrophobic region as well of 38 mol% cholesterol containing DPPC unilamellar vesicles. The study has been carried out in the temperature range 25-45 °C. The variation of the order parameter, S, vs temperature of the lipid phase indicates that with this content of cholesterol the characteristic gel liquid crystalline main phase transition of DPPC, normally occurring at T x ~ 41 °C, disappears. When halothane and chloroform are added to the vesicles suspension up to [DPPC]/[anesthetic] molar ratio of 1:1 the main phase transition, as detected with the stearic acid spin label 7(12,3), reappears again and it results down shifted at 7, ~ 35 and 39 °C, respectively. In presence of diethyl ether, instead, the main phase transition is not observable also at the highest concentration of anesthetic used. Moreover, halothane and chloroform affect similarly the hydrophobic core of choles-terol-!-DPPC vesicles which, in turn, results to be different from the action exerted by diethyl ether in the same region. The ESR findings are discussed in terms of competitive effects shown by cholesterol and inhalation anesthetics. Moreover, the interfacial region of CHOL + DPPC vesicles results to be the target of anesthetics. 
  Reference    Z. Naturforsch. 43c, 264—268 (1988); received August 12/November 24 1987 
  Published    1988 
  Keywords    Cholesterol, DPPC, Inhalation Anesthetics, ESR 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0264.pdf 
 Identifier    ZNC-1988-43c-0264 
 Volume    43 
3Author    BożennaR. Óżycka-Roszak, Hanna PruchnikRequires cookie*
 Title    Influence of Dodecyltrimethylammonium Halides on Thermotropic Phase Behaviour of Phosphatidylcholine/Cholesterol Bilayers  
 Abstract    Effects of dodecyltrimethylammonium chloride (DTAC), dodecyltrimethylammonium bro­ mide (DTAB) and dodecyltrimethylammonium iodide (DTAI) on thermotropic phase behav­ iour of phosphatidylcholine bilayers containing cholesterol as well as on 'H N M R spectra were studied. Two series of experiments were performed. In the first one the surfactants were added to the water phase while in the other directly to the lipid phase (a mixed film from cholesterol, surfactant and phosphatidylcholine was formed). The effects of particular surfactants on the main phase transition temperature, Tm, were more pronounced when added to the lipid phase (2 nd method) than to the water phase (1 st method); the opposite happened when cholesterol was absent (Rözycka-Roszak and Pruchnik 2000, Z. Naturforsch. 55c, 240-244). Furthermore, in the case of the first method the transitions were asymmetrical while in the second method nearly symmetrical. It is suggested that surfactant poor and surfactant rich domains are formed when surfactants are added to the water phase. 
  Reference    Z. Naturforsch. 55c, 753—757 (2000); received March 27/May 11 2000 
  Published    2000 
  Keywords    Counterions, Phosphatidylcholine Bilayers, Cholesterol 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0753.pdf 
 Identifier    ZNC-2000-55c-0753 
 Volume    55 
4Author    D. Urga, N. Ath, D. Har, A. Nil, K. Um Ar, S. InghRequires cookie*
 Title    Pyridinium Chlorochromate Oxidation of Some Steroidal Systems. Regioselective Opening o f Ring 'F ' in Spirostan  
 Abstract    The oxidation of diosgenin (1), cholesterol (2), lanosterol (8), ^-sitosterol (4) and stigma­ sterol (5) with pyridinium chlorochromate furnished the corresponding conjugated and unconjugated ketones i.e., zl5< 6)-3-one and Zl4<5)-3-one. In the case of diosgenin (1), besides the formation of the ketones, regioselective opening of the ring F has also been observed. 
  Reference    (Z. Naturforsch. 32b, 1476—1477 [1977]; received July 26 1977) 
  Published    1977 
  Keywords    Cholesterol, Lanosterol, /3-Sitosterol, Stigmasterol, Pyridinium Chlorochromate 
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 TEI-XML for    default:Reihe_B/32/ZNB-1977-32b-1476.pdf 
 Identifier    ZNB-1977-32b-1476 
 Volume    32 
5Author    BodoC., TagRequires cookie*
 Title    Specific Crystal Chemical Interactions between Carcinogenic Aromatic Compounds and Cholesterol  
 Abstract    Polycyclic aromatic hydrocarbons and aromatic amines exercise a highly specific influence on the crystallization o f cholesterol. The strength o f these non-covalent, presumably epitaxial interactions correlates with the carcinogenic activity o f these substances. The presented results are in support o f the assumption that a specific process o f adsorption and crystallization with cholesterol o f the plasma membrane takes place during the initial phase o f the carcinogenesis by aromatic compounds. 
  Reference    Z. Naturforsch. 46c, 663—6 (1991); received September 6 1988/March 21 1991 
  Published    1991 
  Keywords    Carcinogenic Aromatic Compounds, Epitaxial Adsorption, Cholesterol, Plasma Membrane 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0663.pdf 
 Identifier    ZNC-1991-46c-0663 
 Volume    46 
6Author    Meinrad Boll3, LutzW D Webera, Juliana Planac, Andreas Stampfl3, H.M Gcoa ReductaseRequires cookie*
 Title    In Vivo and in Vitro Studies on the Regulatory Link between 3-Hydroxy-3- methylglutaryl Coenzyme A Reductase and Cholesterol 7«-Hydroxylase in Rat Liver  
 Abstract    The activities of 3-hydroxy-3-methylglutaryl CoA reductase (HM GCoA reductase; E C, rate-limiting enzyme of cholesterol biosynthesis, and cholesterol 7a-hydroxylase (E C, key enzyme of the neutral bile acid synthesis pathway, were measured in the microsomal fraction of rat liver and in rat liver cells to investigate the coordinate regula­ tion of the two pathways. Both enzyme activities exhibited the same diurnal rhythm and responded in a coordinate fashion to fasting or bile acid-feeding (decrease) and to cholestyramine-feeding (increase). Cholesterol-feeding decreased the activity of HMGCoA reductase, increased that of choles­ terol 7a-hydroxylase, and concomitantly increased free cholesterol in microsomes. In an ex vivo setting using primary hepatocytes from animals fed a high cholesterol diet the activity of HM GCoA reductase was initially low and that of cholesterol 7a-hydroxylase was elevated. Release of cholesterol into the medium with ongoing incubation caused H M GCoA reductase activity to increase, and that of cholesterol 7a-hydroxylase to decline. Incubation of hepatocytes with a cholesterol-containing lipoprotein fraction stimulated the activity of cholesterol 7a-hydroxylase, but left HMGCoA reductase activity unaffected. The results confirm the idea of a joint regulation of the two key enzymes of cholesterol metabolism in response to the levels of substrate and metabolites, and support the notion that with respect to bile acid and cholesterol levels, respectively, regulation of HM GCoA reductase activity may be secondary to that of cholesterol 7a-hydroxylase. The in vitro studies supply evidence that the effects of cholesterol and bile acid excess or deficiency are direct and do not involve accessory changes of hormone levels or mediators. 
  Reference    Z. Naturforsch. 54c, 371 (1999); received January 18/March 3 1999 
  Published    1999 
  Keywords    Cholesterol 7a-Hydroxylase, Enzyme Regulation, Cholesterol, Rat Liver 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0371.pdf 
 Identifier    ZNC-1999-54c-0371 
 Volume    54 
7Author    Peter Flesch, Michael SchaeferRequires cookie*
 Title    Untersuchungen zur Sterinsynthese des Ascomyceten Aureobasidium (Dematium) pull ul ans  
 Abstract    Studies of the Sterolsynthesis in the Fungus A u reo b a sid iu m (D em a tiu m) pullulans Isolation and identification of sterols from culture extracts of the fungus Aureobasidium (Dematium) pullulans yielded metabolites with key function in sterol synthesis. By proof of squalene, lanosterol/dihydrolanosterol, desmosterol, ergosterol, cholesterol, stigmasterol and ß-sitosterol it was possible to work out a general synthesis-scheme for the building-up of sundry sterols in Ascomycetes. 
  Reference    Z. Naturforsch. 40c, 309—312 (1985); received August 15 1984/January 18 1985 
  Published    1985 
  Keywords    Mycosterols, Lanosterol, Desmosterol, Cholesterol, Stigmasterol, ß-Sitosterol, Aureobasidium (Dematium) pullulans 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0309.pdf 
 Identifier    ZNC-1985-40c-0309 
 Volume    40 
8Author    AneliaG. Dobrikova, NikolaiP. Tuparev, Iwelina Krasteva, MiraH. Busheva, MayaY. VelitchkovaRequires cookie*
 Title    Artificial Alterations of Fluidity of Pea Thylakoid Membranes and Its Effect on Energy Distribution between Both Photosystems  
 Abstract    Two different membrane perturbing agents -cholesterol and benzyl alcohol were applied to modify the fluidity of pea thylakoid membranes. Well pronounced decrease of membrane fluidity was observed upon increasing of cholesterol added. Rigification o f thylakoid m em ­ branes was accompanied by reduction of cation-induced increase of light-scattering intensity and results in lower extent of increase of spillover upon cation induced stack-unstack transi­ tion. Thylakoid membranes, treated with local anesthetic benzyl alcohol, showed an increase of membrane fluidity, but approximately the same behavior as untreated thylakoids in respect to the light scattering intensity dependence on cation concentration. U pon stack-unstack transition the observed changes of energy distribution for benzyl alcohol treated thylakoids are similar to those for controls. The data are discussed in terms of influence of membrane fluidity on lateral reorganization of pigment-protein complexes. 
  Reference    Z. Naturforsch. 52c, 475 (1997); received February 6/April 8 1997 
  Published    1997 
  Keywords    Pisum sativum L Ran /, Benzyl A lcohol, Cholesterol, Energy Distribution, Thylakoid Membrane Fluidity 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0475.pdf 
 Identifier    ZNC-1997-52c-0475 
 Volume    52