| | 1 | Author
| KatherineE. Steinback, Klaus Pfister, CharlesJ. Amtzen | Requires cookie* | | | Title
| Trypsin-Mediated Removal of Herbicide Binding Sites within the Photosystem II Complex  | | | | Abstract
| Trypsin treatment of isolated chloroplast thylakoids resulted in a step-wise modification o f surface exposed membrane polypeptides. Early effects o f the protease action resulted in a decrease in inhibitory activity of atrazine, diuron, pyrazon, and bromacil, but an initial increase in the activity of bromnitrothymol and dinoseb. Direct measurements o f atrazine binding demonstrated that decreased inhibitory activity corresponded to a decreased binding affinity in the treated membranes. Longer term effects o f trypsin caused removal of atrazine binding sites and a concomitant block o f electron transport chains. The data are consistent with a concept that the traizine receptor protein is a component o f the electron transport chain which is successively degraded in two or more steps by protease attack. Polyacrylamide gel electrophoresis of trypsin-treated membranes and sub-membrane fragments derived from these membranes revealed that several polypeptides are membrane surface exposed. The involvement o f a 32000 dalton polypeptide in creating the atrazine binding site is discussed. | | | |
Reference
| Z. Naturforsch. 36c, 98 (1981); received September 221980 | | | |
Published
| 1981 | | | |
Keywords
| Photosynthesis, Atrazine, Diuron, Chloroplast Membranes, Receptor Protein | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-0098.pdf | | | | Identifier
| ZNC-1981-36c-0098 | | | | Volume
| 36 | |
| 2 | Author
| MichaelP. Percival, A. Lan, D. D. Odge | Requires cookie* | | | Title
| Photodynamic Damage to Isolated Chloroplasts: A Possible Model for in vivo Effects of Photosynthetic Inhibitor Herbicides | | | | Abstract
| The breakdown o f chlorophylls, carotenoids, and linolenic acid together with the form ation o f malondialdehyde and ethane was followed in isolated pea chloroplast membranes. Breakdown was enhanced by light, oxygen, D 20 and rose bengal, but retarded by crocetin. The results are discussed in relationship to the role o f singlet oxygen in prom oting dam age in vivo. | | | |
Reference
| Z. Naturforsch. 39c, 482 (1984); received Decem ber 5 1983 | | | |
Published
| 1984 | | | |
Keywords
| Chloroplast Membranes, Lipid Peroxidation, Singlet O xygen, H erbicide D am age, Chloroplast Pigments | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/39/ZNC-1984-39c-0482.pdf | | | | Identifier
| ZNC-1984-39c-0482 | | | | Volume
| 39 | |
|
