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'Chlorogenic Acid Esterase' in keywords
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1980 (1)
1Author    B. Schöbel, W. PollmannRequires cookie*
 Title    Isolation and Characterization of a Chlorogenic Acid Esterase from Aspergillus niger  
 Abstract    The isolation and characterization o f a specific chlorogenic acid esterase is described. The en­ zyme activity is measured by determination of the hydrolysis product caffeic acid. The enzyme had been concentrated by means o f ultrafiltration and column-chromatography. The pH-and tempe­ rature optimum were 6.5 and 45 °C respectively. Divalent cations were not required for the en­ zyme activity. As other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. TTie Ä Tm-value is 0.70 mM chlorogenic acid, the molecular weight 240000. The described enzyme is specific for chlorogenic acid. On the other hand a typical unspecific esterase like the pig liver esterases does not split chloro­ genic acid. The isoelectric focusing reveals several isoenzymes o f chlorogenase within a pl-range o f 4 .0 -4 .5 . 
  Reference    Z. Naturforsch. 35c, 209 (1980); received November 20 1979/January 17 1980 
  Published    1980 
  Keywords    Chlorogenic Acid Esterase, Aspergillus niger, High Performance Thin Layer Chromatography 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0209.pdf 
 Identifier    ZNC-1980-35c-0209 
 Volume    35