ZfN: Search Results

1	Author	Gertrud Wiese, Doris Hugo-Wissemann, HansJ. Grambow	Requires cookie*
	Title	An Improved Procedure for the Quantitative Estimation of the Rust Fungus in Infected Plant Tissue
	Abstract	A quantitative estimation of the rust fungus in infected wheat leaves was possible following enzymatic hydrolysis of the chitin and colorimetric determination of the N-acetyl-glucosamine released. The apparently complex cell wall structure of the fungal structures made it necessary, how ever, to use an enzyme mixture of chitinase and cellulase in order to make accessible the chitin of the cell wall for diges tion by chitinase. In applying this method the measurement is not appreciably influenced by already formed uredio-spores as would otherwise be the case were the chitin to be determined on the basis of glucosamine after chemical hydrolysis.
	Reference	Z. Naturforsch. 41c, 1127—1130 (1986); received July 17. 1986
	Published	1986
	Keywords	Rust Fungus, Chitin, N-Acetylglucosamine
	Similar Items	Find
	DEBUG INFO
	TEI-XML for	default:Reihe_C/41/ZNC-1986-41c-1127_n.pdf
	Identifier	ZNC-1986-41c-1127_n
	Volume	41

2	Author	OmP. Agrawal, Klaus Scheller	Requires cookie*
	Title	Sclerotization of Insect Cuticle in a Cell-Free System
	Abstract	Incubation o f deproteinized larval cuticle (chitin flakes) with purified arylphorin (calli-phorin) or larval haemolymph o f Calliphora vicina resulted in the formation o f a chitin-protein complex. Enzymatic oxidation o f N-ß-alanyldopam ine (N D A B) in the presence o f chitin flakes or the chitin-protein com plex, resulted in various degrees o f cross-linking o f N B A D -quinone formed with chitin. This study confirms the involvement o f arylphorin in the process o f quinone tanning o f insect cuticle.
	Reference	Z. Naturforsch. 45c, 129—131 (1990); received October 4 1989
	Published	1990
	Keywords	Arylphorin, Chitin, Cuticle, Sclerotization, Calliphora
	Similar Items	Find
	DEBUG INFO
	TEI-XML for	default:Reihe_C/45/ZNC-1990-45c-0129.pdf
	Identifier	ZNC-1990-45c-0129
	Volume	45

3	Author	Kohki Akiyamaa, Kazuyoshi Kawazua, Akio Kobayashib	Requires cookie*
	Title	Partially N-Deacetylated Chitin Oligomers (Pentamer to Heptamer) are Potential Elicitors for (+)-Pisatin Induction in Pea Epicotyls
	Abstract	Oligomers (dimer to heptamer) of chitosan and their partially/completely N-acetylated derivatives were examined for (+)-pisatin-inducing activities in pea epicotyl elicitor assay at concentrations ranging from 6.25 to 100 j.ig/ml. The structures of the oligomers were analyzed by 'H NM R and M A L D I TOF MS spectrometers. The chitosan oligomers from dimer to tetramer were inactive, while the pentamer exhibited a moderate activity at 100 \|ig/ml. The hexamer showed a pronounced activity at 100 \|ag/ml, whereas the oligomer had no activity at the higher concentrations. Although the chitosan heptamer induced a moderate amount of (+)-pisatin (ca. 60 j.ig/g fr. wt.) at 50 (.ig/ml, the oligomer did not elicit pisatin formation at 100 \|ig/ml. The dimer to tetramer of both partially N-deacetylated chitin (DAC) and chitin were totally inactive. The DAC pentamers (d.a. 31%, 52%), DAC hexamers (d.a. 26%, 41%), and DAC heptamers (d.a. 26%, 36%) exhibited significant elicitor activities at lower concen trations (6.25-25 [.ig/ml) than the native chitosan oligomers. The chitin pentamer to heptamer had weak or no activity. Both glucosamine and N-acetylglucosamine were inactive in the assay. These facts suggest that the DAC pentamer to heptamer may act as elicitors for pisatin induction in pea-fungus interactions.
	Reference	Z. Naturforsch. 50c, 391—397 (1995); received January 4/January 15 1995
	Published	1995
	Keywords	Elicitor, Chitosan, Chitin, Phytoalexin, Pisum sativum
	Similar Items	Find
	DEBUG INFO
	TEI-XML for	default:Reihe_C/50/ZNC-1995-50c-0391.pdf
	Identifier	ZNC-1995-50c-0391
	Volume	50