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1994 (1)
1984 (1)
1Author    D. Ieter, Strack An, M. Aria Bokern, Jochen Berlin An D, Sabine SiegRequires cookie*
 Title    Metabolie Activity of Hydroxycinnamic Acid Glucose Esters in Cell Suspension Cultures of Chenopodium rubrum  
 Abstract    Cell suspension cultures o f Chenopodium rubrum accumulate high amounts of metabolically active glucose esters of ^?-coumaric and ferulic acid. Pulse-labelling experiments using [14C]phe-nylalanine and application o f the phenylalanine ammonia-lyase (PAL) inhibitor L-a-aminooxy-/3-pnenylpropionic acid (AOPP) revealed that the glucose esters are subject to high turnover. A considerable portion o f the ferulic acid was found as an insoluble component, probably bound to cell wall material. Application of m-fluoro-DL-tyrosine (MFT), an effective inhibitor o f tyrosine biosynthesis via L-arogenate, markedly increased the amount of 1-p-coumaroyl-and 1-feruloyl-glucose accumulated. 
  Reference    Z. Naturforsch. 39c, 902—907 (1984); received May 16 1984 
  Published    1984 
  Keywords    Chenopodium rubrum, Cell Culture, Hydroxycinnamic Acid, Glucose Ester, Betalain 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0902.pdf 
 Identifier    ZNC-1984-39c-0902 
 Volume    39 
2Author    Jutta Thiem, Wolfgang BarzRequires cookie*
 Title    Photoautotrophic Chenopodium rubrum Cell Suspension Cultures Resistant against Photosynthesis-Inhibiting Herbicides I. Selection and Characterization  
 Abstract    For establishing metribuzin-resistant, photoautotrophic Chenopodium rubrum cell cul­ tures plated cells, callus cultures or suspension cultures were subjected to selection proce­ dures. The most effective procedure was the stepwise increase in the concentration of the herbicide from 0.01 ^im to 10 ^im in suspension cultures, which resulted in the isolation of eight different metribuzin-resistant photoautotrophic cell lines. Conjugation metabolism or a decrease in the uptake and translocation of the selective agent were not responsible for resist­ ance, which was stable in the absence of the inhibitor over numerous growth cycles. Meas­ urements of the photosynthetic electron transport, analyses of fluorescence induction kinetics and determination of the binding properties of 14C-labelled metribuzin to isolated thylakoids indicated that resistance of the cell lines is based on an alteration in the photosystem II her­ bicide-binding protein (D 1 protein). RFLP analysis of the psbA gene of the eight resistant cell lines demonstrated that none of them possess an amino acid exchange in position 264 of the D 1 protein leading to altered herbicide-binding properties. 
  Reference    Z. Naturforsch. 49c, 186—194 (1994); received June 25/December 161993 
  Published    1994 
  Keywords    Herbicide Resistance, Selection Mechanism, Photoautotrophic Cell Culture, Chenopodium rubrum 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0186.pdf 
 Identifier    ZNC-1994-49c-0186 
 Volume    49