| | 1 | Author
| Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V R | Requires cookie* | | | Title
| A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  | | | | Abstract
| Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi tates development of a rapid methodology to characterize deviations from the wildtype se quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. | | | |
Reference
| Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 | | | |
Published
| 1990 | | | |
Keywords
| Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album | | | |
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| default:Reihe_C/45/ZNC-1990-45c-0418.pdf | | | | Identifier
| ZNC-1990-45c-0418 | | | | Volume
| 45 | |
| 2 | Author
| P. Solymosi, E. Lehoczki | Requires cookie* | | | Title
| Co-Resistance of Atrazine-Resistant Chenopodium and Amaranthus Biotypes to other Photosystem II Inhibiting Herbicides | | | | Abstract
| Biotypes of Amaranthus retroflexus L., A. hybridus L., A. bouchonii Thell. and Chenopodium album L. insensitive to atrazine were collected from maize monoculture where atrazine had been applied extensively. Atrazine-resistant biotypes of A. retroflexus and A. hybridus showed phen medipham and lenacil co-resistance and atrazine-resistant biotype of C. album showed fenuron co-resistance. An atrazin-resistant biotype of A. bouchonii with co-resistance to diuron was not resistant to fenuron, lenacil and phenmedipham. | | | |
Reference
| Z. Naturforsch. 44c, 119 (1989); received August 22 1988 | | | |
Published
| 1989 | | | |
Keywords
| Atrazine-Resistance, Atrazine and Diuron, Fenuron, Lenacil, Phenmedipham Co-Resistance, Amaranthus bouchonii, A hybridus, A retroflexus, Chenopodium album | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0119.pdf | | | | Identifier
| ZNC-1989-44c-0119 | | | | Volume
| 44 | |
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