| | 1 | Author
| Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V R | Requires cookie* | | | Title
| A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  | | | | Abstract
| Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi tates development of a rapid methodology to characterize deviations from the wildtype se quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. | | | |
Reference
| Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 | | | |
Published
| 1990 | | | |
Keywords
| Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album | | | |
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| default:Reihe_C/45/ZNC-1990-45c-0418.pdf | | | | Identifier
| ZNC-1990-45c-0418 | | | | Volume
| 45 | |
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