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'Chenopodium album' in keywords Facet   Publication Year 1990  [X]
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1990[X]
1Author    Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V RRequires cookie*
 Title    A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  
 Abstract    Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel­ ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi­ tates development of a rapid methodology to characterize deviations from the wildtype se­ quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. 
  Reference    Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 
  Published    1990 
  Keywords    Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0418.pdf 
 Identifier    ZNC-1990-45c-0418 
 Volume    45