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1985 (1)
1983 (1)
1Author    Hans HarmsRequires cookie*
 Title    Uptake and Conversion of Three Different 5-Ring Polycyclic Aromatic Hydrocarbons (PAH s) in Cell Suspension Cultures of Various Chenopodiaceae-Species  
 Abstract    Three 14C-labelled 5-ring PAHs, benzo(a)pyrene, dibenz(a,h)anthracene and perylene were added to various cell suspension cultures o f plants belonging to the Chenopodiaceae family. The differently configurated 5-ring systems varied in uptake and metabolism. The uptake of benzo(a)pyrene was highest in all cell cultures tested whereas the assimilation of dibenz(a,h)an-thracene and perylene was significantly less. The uptake o f benzo(a)pyrene and dibenz(a,h)-anthracene was highest in those cultures which showed the highest increase in biomass. In contrast the uptake of perylene was highest in cultures whose increase in biomass was less. Previous investigations (H. Harms, W. Dehnen and W. Mönch, Z. Naturforsch. 32 c, 3 2 1 -3 2 6 (1977)) showed that benzo(a)pyrene is metabolized to quinones and other oxygenated derivatives by Chenopodium rubrum cell cultures. It was demonstrated now that in further reactions these compounds are incorporated into insoluble fractions. With increasing time o f incubation the amount of radioactivity in the extracted residue increased. The proportion o f oxygenated derivatives formed seems to be correlated with the incorporation of radioactivity into insoluble fractions. Dibenz(a,h)anthracene turned out to be the most stable 5-ring system whereas perylene is metabolized to a certain extent but only small amounts o f radioactivity could be detected in the extracted insoluble residue. The experiments showed that plant cell cultures maintain their specific behaviour over long periods. On the other hand each o f the chemical compounds, because of its molecular size and configuration, is subject to its own specific metabolism in plant systems. 
  Reference    Z. Naturforsch. 38c, 382—382 (1983); received December 30 1982/March 2 1983 
  Published    1983 
  Keywords    5-Ring Polycyclic Aromatic Hydrocarbons, Uptake, Metabolism, Plant Cell Suspension Cultures, Chenopodiaceae 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0382.pdf 
 Identifier    ZNC-1983-38c-0382 
 Volume    38 
2Author    Kim Thresh, RagaiK. IbrahimRequires cookie*
 Title    Are Spinach Chloroplasts Involved in Flavonoid O-Methylation?  
 Abstract    Intact spinach chloroplasts, which were isolated on a cushion of Percoll and purified on discon­ tinuous sucrose gradients, were found to adsorb appreciable amounts of high specific activity, cytosolic O-methyltransferases. The latter activity was readily eliminated after washing the chloroplast pellet with 0.1 m phosphate in sucrose gradient buffer and finally with 0 .1% nonidet. Furthermore, the OMT activity of spinach leaves was resolved by chromatography on DEAE-Sepharose CL-6B into caffeic acid (COMT) and flavonoid (FOMT) O-methyltransferase ac­ tivities, and purified to 30-and 50-fold, respectively. The similarities between the FOMT/COMT activity ratios and the methylation patterns of intact chloroplasts and their supernatants, as well as those of purified leaf preparations, suggest non-specific binding of cytosolic OMTs to chloroplast envelopes. It is concluded, therefore, that spinach chloroplasts are not involved in the methylation of phenylpropanoid or flavonoid compounds. These results call attention for the re-evaluation of the role of this organelle in the biosynthesis or accumulation of plant secondary metabolites. 
  Reference    Z. Naturforsch. 40c, 331 (1985); received December 11 1984/February 26 1985 
  Published    1985 
  Keywords    Spinacea oleracea, Chenopodiaceae, Chloroplasts, O-Methyltransferase, Caffeic Acid, Quercetin 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0331.pdf 
 Identifier    ZNC-1985-40c-0331 
 Volume    40