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'Characterization' in keywords Facet   section ZfN Section C  [X]
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1Author    Horst Beier, LotharF. Fecker, Jochen BerlinRequires cookie*
 Title    Lysine Decarboxylase from H afnia alvei: Purification, Molecular Data and Preparation of Polyclonal Antibodies  
 Abstract    The purification and molecular properties of lysine decarboxylase from Hafnia alvei and the preparation of polyvalent antibodies specific for this enzyme are described. The enzyme was purified within two HPLC steps on a TSK G 4000 SW and a MonoQ column to homogeneity. The subunit of the enzyme has a molecular weight of approximately 80.000 d. Under "native" condi­ tions it seems to form aggregates up to ten subunits. Lysine decarboxylase from H. alvei contains one mol pyridoxal phosphate per mol subunit. Antibodies against the lysine decarboxylase were purified by affinity chromatography. 
  Reference    Z. Naturforsch. 42c, 1307—1312 (1987); received June 5. 1987 
  Published    1987 
  Keywords    Hafnia alvei, Enterobacterium, Lysine Decarboxylase Purification, Characterization 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-1307.pdf 
 Identifier    ZNC-1987-42c-1307 
 Volume    42 
2Author    Manfred Wiegand, Karl EiseleRequires cookie*
 Title    Isolation and Characterization of the Androgen Receptor of Murine Preputial Gland  
 Abstract    The androgen receptor of murine preputial gland showed in binding experiments a biphasic saturation curve and a biphasic Scatchard plot. The receptor converted from an 8.5-9 S form to a 4.5—5 S form in high ionic strength buffer. The apparent dissociation constant was K D 0.5 ± 0.2 nM for the 8.5—9 S receptor form. A 6.5—7 S receptor form could be detected in some experiments. The ligand specificity was evaluated by competition experiments: testosterone > androstene-dione > dihydrotestosterone > androstanediol > estradiol > progesterone > dexamethasone. The receptor of murine preputial gland was less stable than the androgen receptor of skeletal muscle of the same mice. 
  Reference    Z. Naturforsch. 43c, 117—125 (1988); received September 25 1987 
  Published    1988 
  Keywords    Murine Preputial Gland, Androgen Receptor, Isolation, Characterization 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0117.pdf 
 Identifier    ZNC-1988-43c-0117 
 Volume    43 
3Author    Nariyuki Ishikura, Zhi-Qing YangRequires cookie*
 Title    UDP-D-Xylose: Flavonol 3-O-Xylosyltransferase from Young Leaves of Euonymus alatus f. ciliato-dentatus  
 Abstract    From the young leaves of Euonymus alatus f. ciliato-dentatus, a novel enzyme, UDP-D-xylose: flavonol 3-O-xylosyltransferase (F 3 X T), catalyzing the transfer o f D-xylose from UDP-D-xylose to the 3 position o f 3,5,7,4'-tetrahydroxyflavone (kaempferol), was detected and purified about 16-fold by precipitation with am m onium sulfate and DEAE-cellulose CC, by which F 3 X T was separated from two coexisting flavonol O-glucosyltransferases (FGT). Thus, F 3 XT was isolated as a soluble enzyme with a pH optim um o f 7.0 in Tris-HCl buffer. The molecular weight o f F 3 X T , which had an isoelectric point at pH 6.1, was estimated by elution from a column o f Sephadex G-100 to be about 48 kDa. The activity o f F 3 X T was stimulated by 14 mM 2-ME and strongly inhibited by 1 mM C u 2+, 1 mM Z n 2+, and various re­ agents that react with sulfhydryl groups. Am ong the substrates tested for F 3 X T , kaempferol was the best. The Km values for kaempferol and UDP-xylose were determined to be 0.83 jim and 25 |iM, respectively. F 3 X T mediated the transfer o f xylose exclusively to the 3-hydroxyl group o f kaempferol. Isorhamnetin, quercetin and fisetin also can function as xylosyl acceptor though less efficiently, but neither the 7-O-glucosides nor the 3-O-glucosides o f kaempferol and quercetin were able to accept D-xylose. Dihydroflavonols were not xylosylated. 
  Reference    Z. Naturforsch. 46c, 1003—1010 (1991); received February 28/June 28 1991 
  Published    1991 
  Keywords    Celastraceae, Euonymus, Flavonol, O-Xylosyltransferase, Characterization 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-1003.pdf 
 Identifier    ZNC-1991-46c-1003 
 Volume    46 
4Author    F. A. Tom Ás-Barberán, F. Ferreres, F. Tom Ás-Lorente, A. OrtizRequires cookie*
 Title    Flavonoids from Apis mellifera Beeswax  
 Abstract    The flavonoids present in beeswax produced in "La Alcarria" region were analyzed by HPLC. Pinocembrin, pinobanksin, pinobanksin 3-acetate, chrysin, galangin and techtochry-sin were detected as the main flavonoid constituents. This is the first detailed report on the flavonoids o f beeswax. These substances are already present when wax scales are secreted by bees. The same flavonoid com pounds were generally present in honey, propolis and Populus nigra bud exudates collected in the same geographical region. These results indicate that bees­ wax flavonoids originate from those o f honey and/or propolis, and suggest that analysis o f beeswax flavonoids could be used as an adjunct in the detection o f beeswax adulterations. 
  Reference    Z. Naturforsch. 48c, 68—7 (1993); received N ovem ber 19 1992/January 1 1993 
  Published    1993 
  Keywords    Beeswax, Apis mellifera, Flavonoids, H oney, Propolis, Botanical Origin, Characterization 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0068.pdf 
 Identifier    ZNC-1993-48c-0068 
 Volume    48