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1986 (1)
1984 (1)
1Author    Michael Kästner, H. Rainer, M. Aurer, Ute Gerlach, Hellmut Renner, JosefH W IsslerRequires cookie*
 Title    A Granulopoiesis Inhibitor Partially Purified from Large-Scale Serum-Free Cultures of Porcine Leukocytes  
 Abstract    A method is described for the partial purification o f a granulom onopoiesis inhibitor derived from 2001-batches equivalent to 1 kg or 2 • 1012 leukocytes o f porcine blood. Serum-free condi­ tioned media were concentrated and separated via am m onium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a m olecular mass o f 500—700 Da and was not thiol-dependent, thus contrasting it with previously-reported granulopoiesis inhibitors. Inhibition o f colony form ation in an in vitro m yeloid stem cell assay using various colony-stimulating factors showed that mature granulocytes and m onocytes were equally affected. Apparent lack o f cytotoxicity was suggested using a new reversibility test. 
  Reference    Z. Naturforsch. 39c, 639 (1984); received June 6 1983 
  Published    1984 
  Keywords    Granulopoiesis Inhibitor, Chalone, Porcine Leukocytes, Large-Scale Isolation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0639.pdf 
 Identifier    ZNC-1984-39c-0639 
 Volume    39 
2Author    H.Peter Matthiessen, H.Rainer MaurerRequires cookie*
 Title    Low Molecular Mass Inhibitors from Calf Thymus Selective for T-Lymphocyte Proliferation  
 Abstract    Endogenous factors preferentially inhibiting T-lympho-cyte proliferation were prepared from the acetone precipi­ tate of a 60% ethanol extract from calf thymus and their biochemical properties examined. By ultrafiltration the strongest lymphocyte-selective inhibition was found in the molecular mass range between 1 and 5 kDa. Fast protein liquid chromatography (FPLC) of this fraction on an anion exchange column eluted the lymphocyte inhibitors at 205—265 mM ammonium acetate. Staining procedures following IEF and TLC suggested, that the inhibitors may not be glycoconjugates or amines but small weakly acidic peptides (< 3 kDa). 
  Reference    Z. Naturforsch. 41c, 1131 (1986); received November 11 1985/July 18 1986 
  Published    1986 
  Keywords    Lymphocyte Proliferation Inhibitors, Calf Thymus, Peptides, Chalone, Colony Formation 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-1131_n.pdf 
 Identifier    ZNC-1986-41c-1131_n 
 Volume    41