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'Chalone' in keywords Facet   section ZfN Section C  [X]
Facet   Publication Year 1984  [X]
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1Author    Michael Kästner, H. Rainer, M. Aurer, Ute Gerlach, Hellmut Renner, JosefH W IsslerRequires cookie*
 Title    A Granulopoiesis Inhibitor Partially Purified from Large-Scale Serum-Free Cultures of Porcine Leukocytes  
 Abstract    A method is described for the partial purification o f a granulom onopoiesis inhibitor derived from 2001-batches equivalent to 1 kg or 2 • 1012 leukocytes o f porcine blood. Serum-free condi­ tioned media were concentrated and separated via am m onium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a m olecular mass o f 500—700 Da and was not thiol-dependent, thus contrasting it with previously-reported granulopoiesis inhibitors. Inhibition o f colony form ation in an in vitro m yeloid stem cell assay using various colony-stimulating factors showed that mature granulocytes and m onocytes were equally affected. Apparent lack o f cytotoxicity was suggested using a new reversibility test. 
  Reference    Z. Naturforsch. 39c, 639 (1984); received June 6 1983 
  Published    1984 
  Keywords    Granulopoiesis Inhibitor, Chalone, Porcine Leukocytes, Large-Scale Isolation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0639.pdf 
 Identifier    ZNC-1984-39c-0639 
 Volume    39