| | 1 | Author
| Michael Kästner, H. Rainer, M. Aurer, Ute Gerlach, Hellmut Renner, JosefH W Issler | Requires cookie* | | | Title
| A Granulopoiesis Inhibitor Partially Purified from Large-Scale Serum-Free Cultures of Porcine Leukocytes  | | | | Abstract
| A method is described for the partial purification o f a granulom onopoiesis inhibitor derived from 2001-batches equivalent to 1 kg or 2 • 1012 leukocytes o f porcine blood. Serum-free condi tioned media were concentrated and separated via am m onium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a m olecular mass o f 500—700 Da and was not thiol-dependent, thus contrasting it with previously-reported granulopoiesis inhibitors. Inhibition o f colony form ation in an in vitro m yeloid stem cell assay using various colony-stimulating factors showed that mature granulocytes and m onocytes were equally affected. Apparent lack o f cytotoxicity was suggested using a new reversibility test. | | | |
Reference
| Z. Naturforsch. 39c, 639 (1984); received June 6 1983 | | | |
Published
| 1984 | | | |
Keywords
| Granulopoiesis Inhibitor, Chalone, Porcine Leukocytes, Large-Scale Isolation | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0639.pdf | | | | Identifier
| ZNC-1984-39c-0639 | | | | Volume
| 39 | |
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