| | 1 | Author
| Komei Washino, Otmar Denk, Wolfram Schnabel | Requires cookie* | | | Title
| O H Radical-Induced Main-Chain Scission of Poly(Ribonucleic Acids) under Anoxic Conditions  | | | | Abstract
| Poly(ribonucleic acids), poly A, poly C and poly U, were irradiated in 0 2-free dilute aqueous solution at pH 7 to 8 with single pulses (50 to 200 ns) of 16MeV electrons. With the aid of Rayleigh light scattering measurements main-chain scission, induced by OH radicals, was observed with the three polynucleotides. From the time dependence of the decrease o f the light scattering intensity (LSI), the existence of two modes of decrease was inferred, indicating at least two different chemical mechanisms were operative in main-chain degradation. Evidence for the assignment o f the slow mode of LSI decrease to the lifetime of a free radical was obtained from quenching experiments with cysteamine. It is noteworthy, that the extent and the lifetime o f LSI decrease are not the same for the three polynucleotides. The differences indicate the influence of the chemical nature o f the bases on main-chain scission. Consequently, it is concluded that OH attack at carbons in 1' and/or 2' position of the ribose moiety contributes essentially to the degradation mechanism. | | | |
Reference
| Z. Naturforsch. 38c, 100 (1983); received August 17/November 11 1982 | | | |
Published
| 1983 | | | |
Keywords
| Poly(Ribonucleic Acids), OH Attack, Chain Breakage, Free Radicals, Time-Resolved Light Scattering | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0100.pdf | | | | Identifier
| ZNC-1983-38c-0100 | | | | Volume
| 38 | |
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