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'Chain Breakage' in keywords Facet   Publication Year 1983  [X]
Facet   section ZfN Section C:Volume 038  [X]
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1Author    Komei Washino, Otmar Denk, Wolfram SchnabelRequires cookie*
 Title    O H Radical-Induced Main-Chain Scission of Poly(Ribonucleic Acids) under Anoxic Conditions  
 Abstract    Poly(ribonucleic acids), poly A, poly C and poly U, were irradiated in 0 2-free dilute aqueous solution at pH 7 to 8 with single pulses (50 to 200 ns) of 16MeV electrons. With the aid of Rayleigh light scattering measurements main-chain scission, induced by OH radicals, was observed with the three polynucleotides. From the time dependence of the decrease o f the light scattering intensity (LSI), the existence of two modes of decrease was inferred, indicating at least two different chemical mechanisms were operative in main-chain degradation. Evidence for the assignment o f the slow mode of LSI decrease to the lifetime of a free radical was obtained from quenching experiments with cysteamine. It is noteworthy, that the extent and the lifetime o f LSI decrease are not the same for the three polynucleotides. The differences indicate the influence of the chemical nature o f the bases on main-chain scission. Consequently, it is concluded that OH attack at carbons in 1' and/or 2' position of the ribose moiety contributes essentially to the degradation mechanism. 
  Reference    Z. Naturforsch. 38c, 100 (1983); received August 17/November 11 1982 
  Published    1983 
  Keywords    Poly(Ribonucleic Acids), OH Attack, Chain Breakage, Free Radicals, Time-Resolved Light Scattering 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0100.pdf 
 Identifier    ZNC-1983-38c-0100 
 Volume    38