| | 1 | Author
| Andrea Feld, Klaus Kobek, HartmutK. Lichtenthaler | Requires cookie* | | | Title
| Inhibition of de novo Fatty-Acid Biosynthesis in Isolated Chloroplasts by Different Antibiotics and Herbicides  | | | | Abstract
| Two natural antibiotics, cerulenin and thiolactomycin, were tested for their inhibitory efficacy on de novo fatty-acid biosynthesis of chloroplasts isolated from oat and spinach seedlings and compared with that of known herbicides. With both antibiotics a strong dose-dependent inhibition of the incorporation of [l-14C]acetate into the fatty-acid fraction of the isolated plastids was detected. The /50-values for the inhibition of acetate incorporation into fatty acids are about 4 fiM in the case of thiolactomycin and about 50 ^im in the case of cerulenin for both mono-and dicotyledonous plants. These values are much higher than those of the particular graminicides cycloxydim and diclofop (0.15 and 0.1 ^im), which were developed to control grass weeds in dicotyledonous crop cultures. | | | |
Reference
| Z. Naturforsch. 44c, 976 (1989); received July 3 1989 | | | |
Published
| 1989 | | | |
Keywords
| Cerulenin, de novo Fatty-Acid Biosynthesis, Isolated Chloroplasts, Thiolactomycin | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/44/ZNC-1989-44c-0976.pdf | | | | Identifier
| ZNC-1989-44c-0976 | | | | Volume
| 44 | |
| 2 | Author
| Bernd Listab, A.Ndrea Golza, Wilhelm Bolandb, H. Artm, K. Lichtenthaler3 | Requires cookie* | | | Title
| Inhibition of de novo Fatty-Acid Biosynthesis in Isolated Chloroplasts by the Antibiotic Cerulenin and Its Synthetic Derivatives | | | | Abstract
| The antibiotic cerulenin was shown to be a potent dose-dependent inhibitor o f de novo fatty-acid biosynthesis in intact isolated chloroplasts o f different plants (measured as [l4C]acetate incorporation into the total fatty-acid fraction). Various chemical derivatives o f cerulenin were synthesized and tested in the chloroplast assay-system o f oat, spinach and pea. M odifica tions o f the hydrocarbon chain o f cerulenin (e.g. tetrahydro-cerulenin and its short-chain c«-2,3-epoxy-4-oxoheptanam ide derivative) decreased the inhibitory activity o f cerulenin, whereas variations o f the epoxy-oxo-am ide structural element led to a com plete loss o f inhibi tion potency. The results indicate that the naturally occurring antibiotic cerulenin is the most active specific inhibitor o f de novo fatty-acid biosynthesis, but the form ation o f the hydroxy-lactam ring seems to be an essential requirement for the inhibitory activity. Those structural analogues o f cerulenin, which can no longer form a hydroxylactam ring, do not possess any inhibitory capacity. | | | |
Reference
| Z. Naturforsch. 47c, 382 (1992); received March 10 1992 | | | |
Published
| 1992 | | | |
Keywords
| Cerulenin, Cerulenin Derivatives, de novo Fatty Acid Biosynthesis, Isolated Chloroplasts | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/47/ZNC-1992-47c-0382.pdf | | | | Identifier
| ZNC-1992-47c-0382 | | | | Volume
| 47 | |
| 3 | Author
| JochenF., H. Rm An N, K. Laus-, Peter Heise | Requires cookie* | | | Title
| Factors Controlling Medium-Chain Fatty Acid Synthesis in Plastids from Maturing Cuphea Embryos | | | | Abstract
| The colorless embryos o f Cuphea wrightii A. Gray accumulate capric (about 30%) and lauric acid (about 50%) in their storage lipids. Fractionation studies show that the capacities for the synthesis o f these medium-chain fatty acids (M C FA) from [ l -l4C]acetate were strictly bound to intact plastids. These, in turn, obligately required the addition o f ATP. ATP could partially be substituted by A DP. Reduction o f the pyridine nucleotide pool, required for opti mum M C FA formation within the plastids, was driven by glucose 6-phosphate. Under these conditions the plastids were capable o f synthesizing M C FA like the intact tissue. The presence o f C oA in the incubation medium induced acyl-CoA formation. The observed accumulation o f unesterified capric and lauric acid in the absence o f CoA suggests that acyl-ACP thioesterase activity is involved in the chain termination. Treatment with cerulenin led to an unexpectedly small reduction o f total fatty acid synthesis while the chain elongation o f capric acid was clearly inhibited. A similar accumulation o f capric acid at the expense o f longer chain fatty acids has been observed after replacing ATP by A D P . These findings implicate that even the condensing enzymes are involved in the control o f chain ter mination. | | | |
Reference
| Z. Naturforsch. 48c, 616 (1993); received December 28 1992/M ay 13 1993 | | | |
Published
| 1993 | | | |
Keywords
| Cuphea, de novo Fatty Acid Synthesis, Plastids (Fatty Acid Synthesis), Cerulenin | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/48/ZNC-1993-48c-0616.pdf | | | | Identifier
| ZNC-1993-48c-0616 | | | | Volume
| 48 | |
|
