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1992 (1)
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1Author    Gerhard Sandmann, IanE. Clarke, PeterM. Bramley, Peter BögerRequires cookie*
 Title    Inhibition of Phytoene Desaturase — the Mode of Action of Certain Bleaching Herbicides  
 Abstract    Assay systems have been developed in order to differentiate betw een the m odes o f action o f certain bleaching herbicides. These include inhibition o f chlorophyll or carotenoid biosynthesis, and initiation o f pigment degradation. H erbicidal com pounds with phytoene desaturase as their primary target site were investigated in a cell-free carotenogenic system from Aphanocapsa. In a comparative study, the structural prerequisites for inh ib ition o f phytoene desaturase were estab­ lished for both benzophenone analogs and various m -phenoxybenzam ides. This inhibitory action o f the latter compounds is enhanced by lipophilic groups with certain steric properties. 
  Reference    Z. Naturforsch. 39c, 443 (1984); received N ovem ber 1 1983 
  Published    1984 
  Keywords    Bleaching Herbicides, Cell-Free System, Carotene Biosynthesis, P hytoene Desaturase, Inhibitors 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0443.pdf 
 Identifier    ZNC-1984-39c-0443 
 Volume    39 
2Author    M. Anfred Steingraber, Hanns Frohnm, Rüdiger HamRequires cookie*
 Title    Light-Dependent Sucrose Synthesis in Cell-Free (Reconstituted) Lysates of Evacuolated Mesophyll Protoplasts  
 Abstract    Oat mesophyll protoplasts were evacuolated by centrifugation on a Percoll gradient and used as starting material for establishing a functional cell-free system. For this purpose evacuolated protoplasts were osm otically lysed. From the resulting homogenate a cytosolic fraction was obtained by silicon oil filtration. This fraction was used to dilute preparations o f lysed evacu­ olated protoplasts in order to reduce the number o f organelles per volume cytosol. The latter was necessary to increase light-dependent oxygen evolution o f the cell-free system. The result­ ing kind o f "reconstituted" system showed light-dependent sucrose formation (about 100 nmol (mg Chi)-1 • h r 1) with bicarbonate as the only substrate. As this property depends on a functional interaction o f chloroplast and cytosolic reactions, this cell-free system appeared to perform essential steps o f partitioning o f C 0 2 between starch and sucrose. Addition o f about 12 (im fructose 2 ,6 -bisphosphate, an inhibitor o f fructose 1,6 -bisphosphatase and an activator o f the PPi-dependent fructose 6 -phosphate phosphotransferase, caused sucrose degradation in the light. Thus, this cell-free system allows both the study o f cytosolic enzyme activities under quasi in vivo conditions and the manipulation o f cellular reaction sequences by plasma mem­ brane-impermeable compounds. 
  Reference    Z. Naturforsch. 45c, 217 (1990); received January 11 1990 
  Published    1990 
  Keywords    Avena sativa, Cell-Free System, Evacuolation, Mesophyll Protoplasts, Sucrose Synthesis 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0217.pdf 
 Identifier    ZNC-1990-45c-0217 
 Volume    45 
3Author    Alfred Baumert, Andrea Porzel, Jürgen Schmidt, D. Etlef GrögerRequires cookie*
 Title    Formation of 1,3-Dihydroxy-N-methylacridone from N-Methylanthraniloyl-CoA and Malonyl-CoA by Cell-Free Extracts of Ruta graveolens  
 Abstract    N -M ethylanthraniloyl-CoA was synthesized via N-succinim idyl N-methylanthranilate and subsequent transesterification with C oA-SH . This com pound was characterized by LSIMS and N M R data. An enzyme preparation from cell suspension cultures o f Ruta graveolens cata­ lyzed the formation o f 1,3-dihydroxy-N-methylacridone from N-methylanthraniloyl-CoA and malonyl-CoA with a pH optimum o f 7.5. 
  Reference    Z. Naturforsch. 47c, 365 (1992); received February 10/April 9 1992 
  Published    1992 
  Keywords    Ruta graveolens, Cell-Free System, Acridone Biosynthesis, N-M ethylanthraniloyl-CoA 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0365.pdf 
 Identifier    ZNC-1992-47c-0365 
 Volume    47