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1976 (1)
1Author    Bernd Schulz-Harder, Ernst-Randolf LochmannRequires cookie*
 Title    Initiation in einem Polyribosomen-abhängigen Protein-synthetisierenden zellfreien System aus Saccharomyces Initiation in a Polyribosome-Dependent Protein-Synthesizing Cell-Free System from Saccharomyces  
 Abstract    A method to prepare polyribosomes from yeasts by using the french-press is described. The highest yield of polyribosomes was derived from late log-phase cells. These polyribosomes, in­ cubated in a cell-free system, were able to reinitiate protein synthesis, which was shown by in­ hibiting aminoacid incorporation by aurintricarboxylic acid, edeine and sodiumfluoride. W e de­ veloped the translational system in order to look for the optimal ion-conditions of a DNA-dependent protein-synthesizing system. W e found out that at the optimal MgCL-concentration (6 mM) protein synthesis was strongly inhibited by Mangan ions which are required for transcription in yeast. If protein-synthesis was carried out with 2 mM and 3 mM M gCU maximal aminoacid incorporation was observed at 2 mM and 1.5 mM M nCl2 . 
  Reference    (Z. Naturforsch. 31c, 169 [1976]; eingegangen am 10. November 1975) 
  Published    1976 
  Keywords    Yeast, Polyribosomes, Cell-Free Protein Synthesis, Antibiotics, Ion-Conditions 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0169.pdf 
 Identifier    ZNC-1976-31c-0169 
 Volume    31