| | 1 | Author
| H. Ans-M, Artin Schiebel | Requires cookie* | | | Title
| A Model Mechanism for the Enzymatic Synthesis of Lupin Alkaloids  | | | | Abstract
| A crude enzyme preparation obtained from cell suspension cultures o f Lupinus polyphyllus cat alyzes the pyruvate dependent conversion o f cadaverine into the tetracyclic lupin alkaloids. As the first reaction product 17-oxosparteine could be identified by gas-liquid chrom atography and mass spectroscopy. In som e experiments sparteine was found additionally. A participation o f di amine oxidase could be ruled out. The cadaverine-pyruvate transaminating enzyme system (17-oxosparteine synthase) catalyzes the formation o f 17-oxosparteine from three cadaverine units without releasing free intermediates. These results are inconsistent with the hypothetical m echa nism thus far formulated for the lupin alkaloid biosynthesis. A new enzym atic model mechanism is proposed regarding both the results o f the enzymatic experiments and those o f the in vivo tracer studies. | | | |
Reference
| Z. Naturforsch. 34c, 704—708 (1979); received June 8 1979 | | | |
Published
| 1979 | | | |
Keywords
| Lupinus polyphyllus, Cell Suspension Cultures, Q uinolizidine Alkaloids, Enzymatic Synthesis, Biosynthetic Model Mechanism | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0704.pdf | | | | Identifier
| ZNC-1979-34c-0704 | | | | Volume
| 34 | |
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