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'Cell Suspension Cultures' in keywords Facet   section ZfN Section C:Volume 044  [X]
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1989 (2)
1Author    G. Bärenwald, B. Schneider, H. R. SchütteRequires cookie*
 Title    Comparative Investigations on the Metabolism of the Herbicide 2-(2,4-Dichlorophenoxy)-propionic Acid in Plants and Cultured Cells of Tomato  
 Abstract    A kadem ie der W issenschaften der D D R , Institut für B iochem ie der Pflanzen, W einberg 3, H alle/ Saale. The m etabolism o f [ l -l4C ]2-(2,4-dichlorophenoxy)-propionic acid was studied in excised plants and cell suspension cultures o f tom ato. It was rapidly taken up and m etabolized by both the plants and the cultured cells. The m etabolites, isolated by extraction with aqueous acetone, separated and purified by TLC and H PLC, were identified by chem ical and spectrometric methods. C onju­ gates with carbohydrates were detected. G lucose, diglucose as the main conjugating m oiety, and triglucose were found as carbohydrate com ponents within the conjugates. Alm ost the same conjugates occurred in plants and cultured cells. 
  Reference    Z. Naturforsch. 44c, 233—236 (1989); received O ctober 24 1988/January 16 1989 
  Published    1989 
  Keywords    H erbicide, M etabolism, C onjugation, Cell Suspension Culture 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0233.pdf 
 Identifier    ZNC-1989-44c-0233 
 Volume    44 
2Author    Jochen Berlin, BerndM. Artin, Jerzy Nowak, LudgerW. Itte+, Victor Wray, D. Ieter Strack+, G. Esellschaft Für, Biotechnologische Forschung, BhM. Ascheroder, W. EgRequires cookie*
 Title    Effects of Permeabilization on the Biotransformation of Phenylalanine by Immobilized Tobacco Cell Cultures  
 Abstract    Im m obilized tobacco cells excreted only a small proportion of their main secondary m etabolite caffeoylputrescine into the culture m edium . Experim ents designed to release more o f the com ­ pound by perm eabilization caused the loss o f caffeoylputrescine, probably by oxidative reactions. M oreover, rather mild treatments with pejm eabilizing agents (e.g. «-propanol) resulted in severe growth inhibition. The ability of perm eabilized cells to form caffeoylputrescine and other hy-droxycinnam oyl conjugates from phenylalanine decreased considerably even when such cells were still able to m etabolize phenylalanine into various ethyl acetate extractable com pounds (e.g. hydroxycinnamic acids and acetophenones). The formation of new biotransformation products suggests that perm eabilized cells could be used as a tool for testing the enzymatic capabilities o f a cell culture. 
  Reference    Z. Naturforsch. 44c, 249 (1989); received D ecem ber 5 1988 
  Published    1989 
  Keywords    Nicotiana tabacum, Cell Suspension Cultures, Imm obilized Cells, Permeabilized Cells, Biotrans­ form ation, Phenylalanine 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0249.pdf 
 Identifier    ZNC-1989-44c-0249 
 Volume    44