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'Cell Suspension Cultures' in keywords Facet   section ZfN Section C:Volume 041  [X]
Facet   Publication Year 1986  [X]
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1Author    KarlDietrich Hauffe, KlausH. Ahlbrock, D. Ierk ScheelRequires cookie*
 Title    Elicitor-Stimulated Furanocoumarin Biosynthesis in Cultured Parsley Cells: S-Adenosyl-L-Methionine:BergaptoI and S-Adenosyl-L-Methionine: Xanthotoxol O-Methyltransferases  
 Abstract    Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean patho­ gen, P hytophthora m egasperm a f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-L-methionine: xanthotoxol and S-adenosyl-L-m ethionine: bergaptol O-methyltransferases. The products, xanthotoxin and bcrgapten, were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells. The latter enzyme also catalyzed the methylation of 5-hydroxyxanthotoxin to isopimpinellin, another major coumarin derivative in this system. The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products. No activity was detectable in untreated cells. Activity increases of the O-methyltransferases occurred a few hours later than those of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase. 
  Reference    Z. Naturforsch. 41c, 228 (1986); received October 14. 1985 
  Published    1986 
  Keywords    birthday Furanocoumarins, Phytoalexins, O-Methyltransferases, Fungal Elicitor, Cell Suspension Cultures 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-0228.pdf 
 Identifier    ZNC-1986-41c-0228 
 Volume    41 
2Author    Karl-Heinz Pawelka, Joachim StöckigtRequires cookie*
 Title    Indole Alkaloids from Ochrosia elliptica Plant Cell Suspension Cultures  
 Abstract    From cell suspension cultures of Ochrosia elliptica Labill. eight different monoterpenoid indole alkaloids were isolated and comprised three alkaloid classes viz. Corynanthe/Heteroyohimbine (tetrahydroalstonine, cathenamine, pleiocarpamine and two methoxylated heteroyohimbines), Strychnos (norfluorocurarine), Apparicine (apparicine and epchrosine). A ll these compounds except the Strychnos alkaloid are biogenetically closely related to the known alkaloids occurring in Ochrosia plants, although such alkaloids have not previously been detected in O. elliptica. Epchrosine has not before been described as a natural product. 
  Reference    Z. Naturforsch. 41c, 381 (1986); received November 7 1985 
  Published    1986 
  Keywords    Ochrosia elliptica Labill, Apocynaceae, Cell Suspension Cultures, Indole Alkaloid Composition 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-0381.pdf 
 Identifier    ZNC-1986-41c-0381 
 Volume    41 
3Author    Karl-Heinz Pawelka, Joachim StöckigtRequires cookie*
 Title    Major Indole Alkaloids Produced in Cell Suspension Cultures of Rhazya strict a Decaisne  
 Abstract    Eleven main alkaloids were identified from cell suspension cultures of Rhazya stricta grown in 4X-medium for 15 days. The alkaloids comprised the five groups Corynanthe, Strychnos, Ebur-nan, Secodine, and Aspidosperma and can be regarded as being typical Rhazya alkaloids, al­ though the Strychnos alkaloid akuammicine has been isolated for the first time from the genus Rhazya. The most abundant compound was (+)-l,2-dehydroaspidospermidine (15 mg/1 medium) whereas all other constituents were synthesized in about 5 — 10 times lower amounts. More than 15 further alkaloids were formed as minor components which have not yet been identified. Culti­ vated Rhazya cells have been shown to be one of the richest alkaloid sources from apocynaceous cell suspensions. The isolated compounds, because of their structural heterogeneity, cannot be presently arranged into a scheme with coherent biosynthetic sequences. 
  Reference    Z. Naturforsch. 41c, 385 (1986); received December 17 1985 
  Published    1986 
  Keywords    Rhazya stricta Decaisne, Apocynaceae, Cell Suspension Cultures, Monoterpenoid Indole Alkaloids 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-0385.pdf 
 Identifier    ZNC-1986-41c-0385 
 Volume    41