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'Cell Suspension Cultures' in keywords Facet   Publication Year 1992  [X]
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1992[X]
1Author    Ulrike Mackenbrock, Ralph Vogelsang, Wolfgang BarzRequires cookie*
 Title    Isoflavone and Pterocarpan Malonylglucosides and ß -l ,3 -G l u c a n -and Chitin-Hydrolases are Vacuolar Constituents in Chickpea (Cicer arietinum L.)  
 Abstract    Cell suspension cultures of chickpea (Cicer arietinum L.) were used to prepare protoplasts and vacuoles. The vacuolar preparation revealed only slight contaminations of cytoplasmic marker enzymes. H PLC analysis of the vacuolar extract showed that the malonylglucosides of isoflavones, isoflavanones and pterocarpans are exclusively located in the vacuole. Experi­ ments designed to determine the subcellular localization of the isoflavone malonylglucoside: malonylesterase suggest an association of this enzyme with the vacuolar membrane. Finally, a ß-l,3-glucanase and a chitinase with basic isoelectric points were also found to be localized in the chickpea vacuoles. 
  Reference    Z. Naturforsch. 47c, 815—822 (1992); received August 17 1992 
  Published    1992 
  Keywords    Chickpea, Cicer arietinum, Cell Suspension Cultures, Protoplast, Vacuole 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0815.pdf 
 Identifier    ZNC-1992-47c-0815 
 Volume    47