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1981 (1)
1979 (1)
1Author    J. Freise, P. Magerstedt, F. W. SchmidtRequires cookie*
 Title    Die in vitro und in vivo Stabilität des Einschlusses von Methotrexat in negativ geladenen Liposomen nach Sterilfiltration The in vitro and in vivo Stability of the Entrapment of Methotrexate in Negatively Charged Liposomes after Sterile Filtration  
 Abstract    The preparation of sterile artificial liposomes (phosphatidylcholine: cholesterol: dicethylphos-phate = 5 : 5 : l) by Millipore® filtration is described. Conditions for storing liposome entrapped Methotrexate have been found which allow storage of the drug in the entrapped form over a period of at least 4 months. It is shown that intravenously injected liposome entrapped Metho­ trexate is retained for at least 15 hours in the particulate form. During this time no free Metho­ trexate is detectable in the blood. 
  Reference    Z. Naturforsch. 34c, 114 (1979); eingegangen am 16. August/10. November 1978 
  Published    1979 
  Keywords    Methotrexate, Liposomes, Endocytosis, Cell Membrane 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0114.pdf 
 Identifier    ZNC-1979-34c-0114 
 Volume    34 
2Author    U. Zimmermann, J. Vienken, G. PilwatRequires cookie*
 Title    Rotation of Cells in an Alternating Electric Field: the Occurrence of a Resonance Frequency  
 Abstract    Cells suspended in a low-conducting medium were exposed to an alternating electric field whose frequency was altered between 1 kHz and 2 MHz. A resonance frequency was observed at which all suspended cells rotated about an axis normal to the field lines (when the electric field strength was larger than a threshold value of about 400 V/cm). This resonance frequency varied from species to species o f cells (mesophyll protoplasts of Avena sativa = 2 0 -4 0 kHz, human erythrocytes and ghost cells = 8 0 -1 0 0 kHz, yeast cells = 140-180 kHz, Friend cells = 3 0 -4 0 kHz, at room temperature). The resonance frequency o f cell rotation was observed only under specific experimental conditions which excluded interference by reversible electrical breakdown of cell membranes and by gravitational forces. Glutardialdehyde fixed and heated cells exhibited no rotation in the frequency and field range investigated. The phenomenon o f rotation is discussed in terms 
  Reference    Z. Naturforsch. 36c, 173—177 (1981); received October 22 1980 
  Published    1981 
  Keywords    Electric Field, Cell Rotation, Dipole, Cell Membrane, Resonance Frequency 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0173.pdf 
 Identifier    ZNC-1981-36c-0173 
 Volume    36