1 | Author | Bruno Wolters, U. Do Eilert | Requires cookie* |
Title | Acridonepoxidgehalte in Kalluskulturen von Ruta graveolens und ihre Steigerung durch Mischkultur mit Pilzen.* Accumulation of Acridone-Epoxides in Callus C ultures o f Ruta graveolens Increased by Coculture with Not Host-Specific Fungi ![]() | ||
Abstract | The classic attempt to find optimum culture conditions lead to hormonautotrophic cultures and to dark grown cultures. We made the new attempt to rise the yield o f rutacridone-epoxides by co culture with not hostspecific fungi. Conditions were chosen that only diffusible elicitors could in fluence callus tissue. 14 days of coculture mostly caused reduced acridone-epoxide content, growthrate and up to total disappearance of chlorophyll. Within 3 — 7 days o f coculture some fun gi induced a significant rise of acridone-epoxide content (up to 50-fold). Culture filtrates o f these fungi showed comparable effects. Our results proved the acridone-epoxides to be phytoalexins. The use o f fungal elicitors could be a new method to increase secondary product accumulation in tissue culture. | ||
Reference | Z. Naturforsch. 37c, 575 (1982); received March 22 1982 | ||
Published | 1982 | ||
Keywords | Acridone-Epoxide, Cell Culture, Coculture with Fungi, Phytoalexin, Ruta graveolens | ||
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