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'Cell Culture' in keywords Facet   section ZfN Section C  [X]
Facet   Publication Year 1992  [X]
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1992[X]
1Author    Didier Hallard, Ernst Bleichert, HubertG. Agnon, Satoshi Tahara, Ragai IbrahimRequires cookie*
 Title    Production and Release of Isoflavonoids by Lupin Cell Cultures  
 Abstract    Cell cultures derived from white lupin (Lupinus albus) radicles consist o f a mixture o f friable cell aggregates and finely divided cells. Both types o f tissue accumulate two isoflavonoid aglu-cones, genistein and 2'-hydroxygenistein, their 7-O-glucosides, and their respective 6-, 8-, and 3'-monoprenyl-, as well as the 6,3'-diprenyl derivatives. M ost o f these metabolites are differen­ tially released in the culture medium. The relative am ounts o f aglucones, glucosides and preny-lated derivatives that are produced by the aggregates and cells, as well as those excreted into the medium, are discussed in relation to culture growth. 
  Reference    Z. Naturforsch. 47c, 346 (1992); received December 23 1991 
  Published    1992 
  Keywords    Lupinus albus, Fabaceae, Cell Culture, Isoflavonoid Production, Genistein 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0346.pdf 
 Identifier    ZNC-1992-47c-0346 
 Volume    47 
2Author    Christine Gareis, Christiane Rivero, Ingolf Schuphan, Burkhard SchmidtRequires cookie*
 Title    Plant Metabolism of Xenobiotics. Comparison of the Metabolism of 3.4-Dichloroaniline in Soybean Excised Leaves and Soybean Cell Suspension Cultures  
 Abstract    Excised leaves and cell suspension cultures of soybean (Glycine m ax L.) were incubated with [UL-14C]-3,4-dichloroaniline. The compound was almost completely metabolized after 48 h in both systems; it was apparent that the major detoxification pathways present in the excised leaves were also present in the cultured cells. Besides considerable amounts of insoluble resi­ dues, the N-glucosyl and the N-malonyl conjugates of 3,4-dichloroaniline, and a yet unknown metabolite was formed in the excised leaves; tentatively the latter was identified with the 6'-0-malonylester of N-glucosyl-3,4-dichloroaniline. The cell suspension cultures produced predominantly the N-malonyl conjugate, besides negligible amounts of the N-glucosyl conju­ gate, and insoluble residues; the majority of the N-malonyl compound was excreted into the medium. It was shown, that the metabolism of 3,4-dichloroaniline in excised leaves and cell suspension cultures of soybean was directed towards different end products: the excised leaves were able to make extensive use of cell wall structures as a deposit for xenobiotic bound resi­ dues, resembling plants in this respect; lack of these structures in the cultured soybean cells resulted in a soluble end product, namely the N-malonyl conjugate. 
  Reference    Z. Naturforsch. 47c, 823—829 (1992); received August 5 1992 3. 
  Published    1992 
  Keywords    4-Dichloroaniline, Metabolism, Soybean, Cell Culture, Excised Leaves 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0823.pdf 
 Identifier    ZNC-1992-47c-0823 
 Volume    47