| | 1 | Author
| Didier Hallard, Ernst Bleichert, HubertG. Agnon, Satoshi Tahara, Ragai Ibrahim | Requires cookie* | | | Title
| Production and Release of Isoflavonoids by Lupin Cell Cultures  | | | | Abstract
| Cell cultures derived from white lupin (Lupinus albus) radicles consist o f a mixture o f friable cell aggregates and finely divided cells. Both types o f tissue accumulate two isoflavonoid aglu-cones, genistein and 2'-hydroxygenistein, their 7-O-glucosides, and their respective 6-, 8-, and 3'-monoprenyl-, as well as the 6,3'-diprenyl derivatives. M ost o f these metabolites are differen tially released in the culture medium. The relative am ounts o f aglucones, glucosides and preny-lated derivatives that are produced by the aggregates and cells, as well as those excreted into the medium, are discussed in relation to culture growth. | | | |
Reference
| Z. Naturforsch. 47c, 346 (1992); received December 23 1991 | | | |
Published
| 1992 | | | |
Keywords
| Lupinus albus, Fabaceae, Cell Culture, Isoflavonoid Production, Genistein | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0346.pdf | | | | Identifier
| ZNC-1992-47c-0346 | | | | Volume
| 47 | |
| 2 | Author
| Christine Gareis, Christiane Rivero, Ingolf Schuphan, Burkhard Schmidt | Requires cookie* | | | Title
| Plant Metabolism of Xenobiotics. Comparison of the Metabolism of 3.4-Dichloroaniline in Soybean Excised Leaves and Soybean Cell Suspension Cultures | | | | Abstract
| Excised leaves and cell suspension cultures of soybean (Glycine m ax L.) were incubated with [UL-14C]-3,4-dichloroaniline. The compound was almost completely metabolized after 48 h in both systems; it was apparent that the major detoxification pathways present in the excised leaves were also present in the cultured cells. Besides considerable amounts of insoluble resi dues, the N-glucosyl and the N-malonyl conjugates of 3,4-dichloroaniline, and a yet unknown metabolite was formed in the excised leaves; tentatively the latter was identified with the 6'-0-malonylester of N-glucosyl-3,4-dichloroaniline. The cell suspension cultures produced predominantly the N-malonyl conjugate, besides negligible amounts of the N-glucosyl conju gate, and insoluble residues; the majority of the N-malonyl compound was excreted into the medium. It was shown, that the metabolism of 3,4-dichloroaniline in excised leaves and cell suspension cultures of soybean was directed towards different end products: the excised leaves were able to make extensive use of cell wall structures as a deposit for xenobiotic bound resi dues, resembling plants in this respect; lack of these structures in the cultured soybean cells resulted in a soluble end product, namely the N-malonyl conjugate. | | | |
Reference
| Z. Naturforsch. 47c, 823—829 (1992); received August 5 1992 3. | | | |
Published
| 1992 | | | |
Keywords
| 4-Dichloroaniline, Metabolism, Soybean, Cell Culture, Excised Leaves | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0823.pdf | | | | Identifier
| ZNC-1992-47c-0823 | | | | Volume
| 47 | |
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