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'Cell Culture' in keywords Facet   Publication Year 1990  [X]
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1Author    HildegardM. Aria, W. Arneck, H. Anns, Ulrich SeitzRequires cookie*
 Title    3 ß-Hydroxysteroid Oxidoreductase in Suspension Cultures of Digitalis lanata EH RH  
 Abstract    A 3 ß-hydroxysteroid oxidoreductase was isolated and characterized in the m icrosomes o f Digitalis lanata cell cultures. The enzyme catalyzes the conversion o f 5a-pregnane-3,20-dione to 5a-pregnan-3 ß-ol-20-one and requires N A D (P)H 2. The enzyme was found to have a pH optimum o f 8.0. The reaction had an optimum incubation temperature o f 25 °C with linear reduction for the first 4 h, reaching maximum enzyme activity after 7 h. Substrate kinetics for 5a-pregnane-3,20-dione and N A D P H 2 resulted in apparent A^-values o f 1 8 .5 -2 0 (iM for 5a-pregnane-3,20-dione and 5 0 -1 2 0 |iM for the co-substrate N A D P H ,. In order to localize 3 ß-hydroxysteroid oxidoreductase differential centrifugation as well as linear sucrose density gradient centrifugation were performed. The results obtained lead to the conclusion that 3 ß-hydroxysteroid oxidoreductase is not associated with a single cell compartment, but con­ sists o f a major soluble part and a markedly smaller part o f endoplasmic reticulum-associated activity. 
  Reference    Z. Naturforsch. 45c, 963 (1990); received July 16. 1990 
  Published    1990 
  Keywords    Cell Cultures, Digitalis lanata, 3 ß-Hydroxysteroid Oxidoreductase, Steroids 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0963.pdf 
 Identifier    ZNC-1990-45c-0963 
 Volume    45 
2Author    Joachim Bollmann, Klaus HahlbrockRequires cookie*
 Title    Timing of Changes in Protein Synthesis Pattern in Elicitor-Treated Cell Suspension Cultures of Parsley ( Petroselinum crispum)  
 Abstract    The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methionine-labelled parsley cells (Petroselinum crispum) was analyzed by two-dimensional gel electropho­ resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un­ responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap­ plication; two other groups were strongly reduced in their rates o f synthesis either early or late after elicitor application; and one group was not appreciably affected at all. The elicitor-in-duced changes altered the total protein com position considerably. A few selected, induced pro­ teins were functionally identified. These included two early induced enzymes, phenylalanine amm onia-lyase (PAL) and 4-coum arate:C oA ligase (4C L), and a late induced enzyme, a ber-gaptol O-methyltransferase (BM T) which is specifically involved in the biosynthesis o f furano-coumarin phytoalexins. The biological significance o f the observed differential timing o f changes in protein synthesis rates is discussed. 
  Reference    Z. Naturforsch. 45c, 1011 (1990); received May 30 1990 
  Published    1990 
  Keywords    Cell Culture, Elicitor, Petroselinum crispum, Protein Synthesis, Two-Dim ensional Gel Electrophoresis 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-1011.pdf 
 Identifier    ZNC-1990-45c-1011 
 Volume    45