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'Cell Culture' in keywords Facet   Publication Year 1984  [X]
Facet   section ZfN Section C:Volume 039  [X]
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1Author    D. Ieter, Strack An, M. Aria Bokern, Jochen Berlin An D, Sabine SiegRequires cookie*
 Title    Metabolie Activity of Hydroxycinnamic Acid Glucose Esters in Cell Suspension Cultures of Chenopodium rubrum  
 Abstract    Cell suspension cultures o f Chenopodium rubrum accumulate high amounts of metabolically active glucose esters of ^?-coumaric and ferulic acid. Pulse-labelling experiments using [14C]phe-nylalanine and application o f the phenylalanine ammonia-lyase (PAL) inhibitor L-a-aminooxy-/3-pnenylpropionic acid (AOPP) revealed that the glucose esters are subject to high turnover. A considerable portion o f the ferulic acid was found as an insoluble component, probably bound to cell wall material. Application of m-fluoro-DL-tyrosine (MFT), an effective inhibitor o f tyrosine biosynthesis via L-arogenate, markedly increased the amount of 1-p-coumaroyl-and 1-feruloyl-glucose accumulated. 
  Reference    Z. Naturforsch. 39c, 902—907 (1984); received May 16 1984 
  Published    1984 
  Keywords    Chenopodium rubrum, Cell Culture, Hydroxycinnamic Acid, Glucose Ester, Betalain 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0902.pdf 
 Identifier    ZNC-1984-39c-0902 
 Volume    39 
2Author    Helmut Heinle, Gerhard Sigg, Amo Reich, Klaus-Ulrich ThiedemannRequires cookie*
 Title    Metabolie Effects of Direct Current Stimulation on Cultured Vascular Smooth Muscle Cells  
 Abstract    Vascular smooth muscle cells from rabbit arteries were grown in tissue culture and stimulated by DC impulses (1 mA, 1 V, 10 Hz, 1 ms/imp). Scanning microscopic examination disclosed that in stimulated cultures the cell surface was enlarged by numerous microvilli. This was interpreted as being indicative of an increase in cell activity. Cellular metabolism was character­ ized by analyzing the incubation medium for glucose, glutamate/glutamine, and lactate. When compared to unstimulated controls, stimulation caused an increase in the uptake of glucose and glutamine as well as an increased lactate production. The enhancing effect on metabolism was prevented when the "calcium antagonist" verapamil was present (5 x 10-6 m). Although the exact mechanism by which D C stimulation influences the cells remains obscure, this finding indicates an important mediating role of Ca2+ ions. 
  Reference    Z. Naturforsch. 39c, 1141—1144 (1984); received August 8 1984 
  Published    1984 
  Keywords    Cell Culture, Direct Electric Stimulation, Metabolie Effects, Calcium Antagonist, Vascular Smooth Muscle 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1141.pdf 
 Identifier    ZNC-1984-39c-1141 
 Volume    39