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'Cell Culture' in keywords Facet   Publication Year 1981  [X]
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1Author    Werner Kohl, Barbara Witte, Gerhard HöfleRequires cookie*
 Title    Alkaloide aus Catharanthus roseus-Zellkulturen, II [1] Alkaloids from Catharanthus roseus Tissue Cultures, II [1]  
 Abstract    From the extract of Catharanthus roseus tissue cultures 16 different indole alkaloids have been isolated belonging to heteroyohimbane, aspidosperma and iboga types. The structures were deduced by UV, NMR and mass spectrometry. Ajmalicine (1) and catharanthine (16) were found to be the major constituents, tetra-hydroalstonine (2), akuammigine (3), 3-isoajmalicine (4), pleiocarpamine (6), akuammiline (6), tabersonine (7), 20-hydroxytabersonine (8), lochnericine (9), horhammericine (10), minovincinine (11), vindolinine (12), 20-epivindolinine (18), vindolinine -Nt -oxide (14) and 20-epivindolinine-Nb-oxide (16) could be determined as minor or trace alkaloids. 
  Reference    Z. Naturforsch. 36b, 1153—1162 (1981); eingegangen am 20. Mai 1981 
  Published    1981 
  Keywords    Indole Alkaloids, Catharanthus roseus, Cell Cultures 
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 TEI-XML for    default:Reihe_B/36/ZNB-1981-36b-1153.pdf 
 Identifier    ZNB-1981-36b-1153 
 Volume    36 
2Author    L. Britsch, W. Heller, H. GrisebachRequires cookie*
 Title    Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley  
 Abstract    Soluble enzyme preparations from irradiated cell suspension cultures of parsley (Petroselinum hortense Hoffm.) catalyse the conversion o f flavanone to flavone, dihydroflavonol and flavonol. These reactions require 2-oxoglutarate, Fe2+ and ascorbate as cofactors. In the presence o f these cofactors conversion of dihydroflavonol to flavonol was also observed. With this system in vitro biosynthesis of radioactive flavone, dihydroflavonol and flavonol from [2-14C]malonyl-CoA and 4-coumaroyl-CoA in good yield and with high specific activity is possible. We postulate that synthesis of flavone and flavonol from flavanone proceeds via 2-hydroxy-and 2,3-dihydroxyflavanone, respectively, with subsequent dehydration. The microsomal fraction of the parsley cells contains an NADPH-dependent flavanone 3'-hydroxylase. 
  Reference    Z. Naturforsch. 36c, 742—750 (1981); received June 3 1981 
  Published    1981 
  Keywords    Flavonoids, Biosynthesis, Dioxygenases, Petroselinum hortense, Cell Cultures 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0742.pdf 
 Identifier    ZNC-1981-36c-0742 
 Volume    36