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1980 (1)
1Author    W. G. Pohl, R. K. Reikenbohm, K. SeuwenRequires cookie*
 Title    The Specificity of Ionophore A 23187 in Cation Transport across Lipid Membranes Studies with Lecithin Vesicles  
 Abstract    Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration o f the divalent cations in the buffer was increased from 1 m M to about 100 m M the cation-concentration inside o f the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux o f various cations m ediated by ionophore A 23187 was m easured and relative transport-rates were determined. The following sequence was obtained: Zn2+ > Mn2+ > Ca2+ > M g2+ > Sr2+ > Ba2+ % L i+ > N a +. The efflux o f Ca2+ increased proportional to the square o f the A 2 3 187-concentration. To lecithin-vesicles containing ethylenediam ine tetraacetate (ED TA) inside different cations were added on the outside. In the presence o f A 23187 divalent cations are transported into the vesicles and bound there by EDTA. During the influx o f M e2+-ions into vesicles a H +-efflux was observed. The resulting pH-decrease was measured. The rate o f the pH -change depended on the M e2+-ion used. The sequence was: Cd2+ > Zn2+ > Ca2+ > M n2+ > M g2+ > Sr2+, Ba2+. 
  Reference    Z. Naturforsch. 35c, 562 (1980); received February 11/M arch 6 1980 
  Published    1980 
  Keywords    Ionophore A 23187, Lecithin Vesicles, Cation Specificity, Proton Antiport 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0562.pdf 
 Identifier    ZNC-1980-35c-0562 
 Volume    35