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1Author    JochenB. Erlin, +., Peter Kiss, D. Ieter, M. Üller-Enoch, D. Ieter Gierse, W. Olfgang Barz, B. Oris JanistynRequires cookie*
 Title    Über den Abbau von Chalkonen und Isoflavonen in pflanzlichen Zellsuspensionskulturen Degradation of Chalcones and Isoflavones in P lant Cell Suspension Cultures  
 Abstract    Cell suspension culture of Phaseolus aureus, Glycine max and Pisum sativum have been used to determine the extent of chalcone and isoflavone catabolism. The A-rings of calcones and isoflavones with both resorcinol and phloroglucinol pattern of sub­ stitution have unequivocally been shown to be degraded as measured by carbon dioxide production The earlier described catabolic pathway of chalcones with the B-ring liberated as a substituted benzoic acid has been verified using another chalcone. With the use of various u C-labelled isoflavones it could be demonstrated that essentially all carbon atoms are introduced into catabolic reactions. Incorporation of 4',7-dihydroxyisoflavone (daidzein) into insoluble polymeric material has been shown to proceed via the 3',4',7-trihydroxyisoflavone. 3'-Hydroxylation and subsequent polymeri­ sation of the orf/io-dihydroxy compound can completely be inhibited by using anaerobic conditions which favour glucoside formation instead. 4'-0-Methylgroups in isoflavones prevent the phenolase catalzyed 3'-hydroxylation and thus the incorporation of isoflavones into polymeric structures. 6,7-Dihydroxy substituted insoflavones when fed to cell cultures are not polymerized by phenolase but are rather converted to glycosides. 
  Reference    (Z. Naturforsch. 29c, 374 [1974]; eingegangen am 1. April 1974) 
  Published    1974 
  Keywords    Chalcones, Isoflavones, Catabolism, Plant Cell Cultures 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0374.pdf 
 Identifier    ZNC-1974-29c-0374 
 Volume    29 
2Author    E. Šimaga, KosRequires cookie*
 Title    Š  
 Abstract    Experiments designed to elucidate the mechanism of uracil degradation by E. coli K12S showed that in contrast to uracil, dihydrouracil — the postulated intermediate of a reductive mechanism — did not stimulate the growth of bacteria as additional source of nitrogen, nor it was cata-bolized to ureido carbon dioxide. However, the chromato­ graphic analysis of dihydrouracil metabolic products, re­ vealed the presence of an enzyme converting dihydrouracil to /?-ureidopropionic acid. Results of growth and biochemi­ cal studies indicated that barbituric acid — the postulated intermediate of an oxidative pathway — is not involved in uracil degradation. 
  Reference    Z. Naturforsch. 33c, 1006 (1978); received August 4 1978 
  Published    1978 
  Keywords    Escherichia coli, Catabolism, Uracil, Dihydrouracil, Barbituric Acid 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-1006_n.pdf 
 Identifier    ZNC-1978-33c-1006_n 
 Volume    33 
3Author    Yuko Fukushima3, Hamako Sasamotob, Shigeyuki Babac, Hiroshi Ashihara3Requires cookie*
 Title    The Effect of Salt Stress on the Catabolism o f Sugars in Leaves and Roots of a Mangrove Plant, Avicennia marina  
 Abstract    Respiration and related aspects of metabolism were investigated in the roots and leaves o f 2-year-old trees of the mangrove plant, Avicennia marina in the presence o f 100, 250 and 500 mM NaCl. The rate of respiration o f leaves increased with increasing concentrations of NaCl in the incubation medium, but respiration of roots was not similarly affected. In order to examine the relative rates of catabolism of glucose by the glycolysis-tricarboxylic acid (T C A) cycle and the oxidative pentose phosphate pathway (P P pathway), we determined the rates o f release of 14C 0 2 from [1-14C]glucose and from [ 6 -14C]glucose in segments of roots and leaves. The ratios of rates (C ^ Q) in roots varied from 0.30 to 0.44, while ratios of 0.85 to 0.99 were obtained when leaves were incubated in the presence o f various concentra­ tions o f NaCl. It appeared that the PP pathway was more involved in sugar catabolism in the roots than in the leaves of A. marina. Uniformaly 14C-labelled sucrose, incubated with segments of roots and leaves for 18 h, was converted to C 0 2, amino acids (mainly glutamine), organic acids (mainly malic acid), sugars and ethanol-insoluble macromolecules. The incorpo­ ration o f radioactivity into most of these components was not significantly affected by NaCl. However, in leaves (but not in roots) the release o f 14C 0 2 from [ U -14C]sucrose was en­ hanced by NaCl at 250 mM and 500 m M , while the rate o f incorporation o f radioactivity into macromolecules was reduced by high concentrations o f NaCl. Incorporation of radioactivity from [ U -14C]sucrose into malic acid was enhanced in both roots and leaves by an increase in the concentration o f NaCl from 100 mM to 500 mM (this concentrations is similar to that in sea water). Independent of the concentration of NaCl, more than half o f the radioactivity in the neutral fraction from leaves was incorporated into an unidentified sugar, while in the same fraction from roots, the radioactivity was associated with glucose, fructose and sucrose. On the basis o f these results, a discussion is presented o f the characteristics of catabolism of sugars in A. marina in relation to salt resistance. 
  Reference    Z. Naturforsch. 52c, 187—192 (1997); received October 29/November 25 1996 
  Published    1997 
  Keywords    Avicennia marina, Grey Mangrove, Respiration, Sucrose, Catabolism 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0187.pdf 
 Identifier    ZNC-1997-52c-0187 
 Volume    52