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'Carotenoids' in keywords Facet   Publication Year 1993  [X]
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1993[X]
1Author    Peter Gölz, Jürgen FeierabendRequires cookie*
 Title    Isoprenoid Biosynthesis and Stability in Developing Green and Achlorophyllous Leaves of Rye (Secale cereale L.)  
 Abstract    Formation o f major prenylquinones and carotenoids was investigated by comparing the incorporation o f [14C]mevalonate into segments o f different age from green and etiolated leaves o f 22 °C-grown rye seedlings (Secale cereale L.) and from 32 °C-grown rye leaves which contained bleached and proplastid-like ribosome-deficient plastids, due to a heat-sensitivity o f 70S ribosome formation. The contents o f plastidic isoprenoids were much lower (between 2 -3 0 %) in the achlorophyllous than in green leaves. In green leaves [l4C]mevalonate incor­ poration into non-polar lipids and into plastoquinone was partially inhibited in the presence o f gabaculin, an inhibitor o f chlorophyll synthesis. However, except for ß-carotene, [,4C]mevalonate incorporation into isoprenoids continuously increased with age also in achlorophyllous etiolated or 32 °C-grown, as in green, leaves and was, except for ß-carotene and plastoquinone, higher in etiolated than in green leaves. In bleached 32 °C-grown leaves [l4C]mevalonate incorporation into all plastidic isoprenoids was strikingly (up to 45-fold) higher than in green control leaves. While degradation o f ß-carotene was greatly enhanced in bleached 32 °C-grown leaves, relative to green control leaves, and could thus compensate for a higher apparent synthesis, chase experiments did not reveal any marked differences o f the turnover o f other isoprenoids. The half times o f plastoquinone, phylloquinone and lutein were in the order o f 2 -3 days. W ithin a 24 h chase period a-tocopherol degradation did not become apparent. U ptake o f [14C]mevalonate and [l4C]isopentenyl pyrophosphate by isolated bleached plastids from 32 °C-grown leaves was much more rapid than by chloroplasts and resulted in higher precursor accumulation within the organelle. While mevalonate incorporation into iso­ prenoid lipids was not detected, isopentenyl pyrophosphate was incorporated into isoprenoid lipids, including plastoquinone. Rates o f incorporation by isolated chloroplasts or bleached plastids were o f similar order. The results illustrate that divergent types o f plastid differentia­ tion are associated with fundamental developmental changes o f the metabolic flow o f iso­ prenoid precursors between different products and compartments and, in particular, with changes o f import into the plastid compartment. 
  Reference    Z. Naturforsch. 48c, 886—8 (1993); received May 14/September 27 1993 
  Published    1993 
  Keywords    Carotenoids, Chlorophyll Form ation, Heat-Bleaching (o f Plastids), Isoprenoids, M evalonic Acid Incorporation 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0886.pdf 
 Identifier    ZNC-1993-48c-0886 
 Volume    48