| 1 | Author | Harald Stransky | Requires cookie* |
| Title | Die quantitative Bestimmung von Chloroplastenpigmenten im picomol-Bereich mit Hilfe einer isochratischen H PLC-M ethode A Method for the Quantitative Estimation of Chloroplast Pigments in the Picomole-Range by Use of a Isocratic HPLC-System  | ||
| Abstract | A method is described for the estimation of the pigment content in chloroplasts by means of H P L C . The method is highly sensitive. Less than one picomole of individual pigments — equivalent to 1 m illigram m e fresh weight of spinach leaves — is needed for quantitative analysis. Analysis time is only 15 min, whereas in routine determination by thin layer chromatography a single sample needs 4 hours to be completed. Moreover the pigments are protected against desintegration by light and air. U sin g a isocratic H P LC -m eth od no equilibration of coloumns is necessary, thus permitting four runs per hour. | ||
| Reference | Z. Naturforsch. 33c, 836 (1978); eingegangen am 16. Oktober. 1978 | ||
| Published | 1978 | ||
| Keywords | Carotenoids, Chloroplast Pigments, H igh Perform ance Liqu id Chromatography | ||
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