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1998 (1)
1984 (1)
1Author    Bdel-B AssetRequires cookie*
 Title    Calcium/Calmodulin Regulated Cell Wall Regeneration in Zea mays Mesophyll Protoplasts  
 Abstract    The composition of newly synthesized cell walls starting from enzymatically isolated Zea mays mesophyll protoplasts was studied. The contents of pectin, cellulose and hemicellulose in addition to the wall-associated proteins were followed in the presence of increasing con­ centrations of calcium ions with or without the calcium channel blockers like lanthanum, lithium ions, verapamil, nifedipine and the calmodulin antagonist trifluperazine. Pectin accu­ mulation was inhibited by all antagonists except lanthanum. Cellulose formation, however, was increased by organic antagonists trifluperazine, verapamil and maximally by nifedipine while it was not affected by the inorganic ions lanthanum or lithium. Hemicellulose accumu­ lated with nifedipine present but significantly decreased by all other antagonists. A dded Ca2+ (5 -1 0 m M) reversed most of the blockers-induced inhibition on pectin and hemicellulose. In the case of cellulose, however, calcium concentration which reversed the inhibitory action of these antagonists was dependent on the antagonist. Starch exhibited little alterations indicat­ ing its minor role in deposition of wall components. Cell wall-associated proteins were nega­ tively affected by lanthanum and verapamil and positively by lithium and nifedipine. G en­ erally, this fraction was found in a negative correlation with pectin levels. Chlorophyll contents were lowered after growth for 48 h; this might be due to repeated cell division. These results are discussed in relation to the mechanism of antagonists and effects of calcium. 
  Reference    Z. Naturforsch. 53c, 33—3 (1998); received November ll/D ecem ber 8 1997 
  Published    1998 
  Keywords    Calcium, Calcium Antagonists, Cell Wall Polysaccharides, Protoplasts, Vicia Faba 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-0033.pdf 
 Identifier    ZNC-1998-53c-0033 
 Volume    53 
2Author    Helmut Heinle, Gerhard Sigg, Amo Reich, Klaus-Ulrich ThiedemannRequires cookie*
 Title    Metabolie Effects of Direct Current Stimulation on Cultured Vascular Smooth Muscle Cells  
 Abstract    Vascular smooth muscle cells from rabbit arteries were grown in tissue culture and stimulated by DC impulses (1 mA, 1 V, 10 Hz, 1 ms/imp). Scanning microscopic examination disclosed that in stimulated cultures the cell surface was enlarged by numerous microvilli. This was interpreted as being indicative of an increase in cell activity. Cellular metabolism was character­ ized by analyzing the incubation medium for glucose, glutamate/glutamine, and lactate. When compared to unstimulated controls, stimulation caused an increase in the uptake of glucose and glutamine as well as an increased lactate production. The enhancing effect on metabolism was prevented when the "calcium antagonist" verapamil was present (5 x 10-6 m). Although the exact mechanism by which D C stimulation influences the cells remains obscure, this finding indicates an important mediating role of Ca2+ ions. 
  Reference    Z. Naturforsch. 39c, 1141—1144 (1984); received August 8 1984 
  Published    1984 
  Keywords    Cell Culture, Direct Electric Stimulation, Metabolie Effects, Calcium Antagonist, Vascular Smooth Muscle 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1141.pdf 
 Identifier    ZNC-1984-39c-1141 
 Volume    39