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'Caffeine Induced Calcium Release' in keywords
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1992 (1)
1Author    Wilhelm Hasselbach, Andrea MigalaRequires cookie*
 Title    Modulation by Ryanodine of Active Calcium Loading and Caffeine Induced Calcium Release of Heavy Sarcoplasmic Reticulum Vesicles  
 Abstract    The effect o f ATP on the calcium release channel in heavy sarcoplasmic reticulum vesicles modulated by ryanodine has been analyzed by monitoring active calcium uptake and caffeine induced calcium release under near physiological conditions. N ative as well as ryanodine reacted vesicles display a complex time course o f calcium uptake resulting in nearly complete exhaustion o f medium calcium when ATP in combination with an ATP-regenerating system, in contrast to ATP alone, or dinitrophenyl phosphate, were used to support calcium uptake. Applying o f dinitrophenyl phosphate as energy yielding substrate, not affecting channel activi­ ty, allowed to estimate the fraction o f light vesicles devoided o f calcium channels contam inat­ ing the heavy preparation as the fraction that stores calcium after the preparation has been treated with channel opening, low concentrations o f ryanodine (1 jim). Calcium uptake by contam inant light vesicles (25%) cannot account for calcium storage, as well as, abolition o f caffeine induced calcium release o f ryanodine treated heavy vesicles. Calcium uptake o f native and ryanodine treated vesicles is accompanied by the uptake o f equivalent amounts o f inor­ ganic phosphate arising from ATP hydrolysis indicating that calcium is mainly stored as cal­ cium phosphate. The momentary capability o f the preparation to accumulate calcium was measured by activating calcium uptake during the calcium storage period with 0.2 m M 45CaCl2 and 4 m M phosphate at short time intervals. A significant increase o f the momentary uptake activity with time was observed being more pronounced for ryanodine treated than for native vesicles indicating that under regenerating conditions, A TP can induce closing o f the native and even more effectively o f the ryanodine modified calcium release channels. 
  Reference    Z. Naturforsch. 47c, 429 (1992); received December 4 1991/March 16 1992 
  Published    1992 
  Keywords    Sarcoplasmic Reticulum, Caffeine Induced Calcium Release, Ryanodine 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0429.pdf 
 Identifier    ZNC-1992-47c-0429 
 Volume    47