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1996 (1)
1985 (1)
1Author    Kim Thresh, RagaiK. IbrahimRequires cookie*
 Title    Are Spinach Chloroplasts Involved in Flavonoid O-Methylation?  
 Abstract    Intact spinach chloroplasts, which were isolated on a cushion of Percoll and purified on discon­ tinuous sucrose gradients, were found to adsorb appreciable amounts of high specific activity, cytosolic O-methyltransferases. The latter activity was readily eliminated after washing the chloroplast pellet with 0.1 m phosphate in sucrose gradient buffer and finally with 0 .1% nonidet. Furthermore, the OMT activity of spinach leaves was resolved by chromatography on DEAE-Sepharose CL-6B into caffeic acid (COMT) and flavonoid (FOMT) O-methyltransferase ac­ tivities, and purified to 30-and 50-fold, respectively. The similarities between the FOMT/COMT activity ratios and the methylation patterns of intact chloroplasts and their supernatants, as well as those of purified leaf preparations, suggest non-specific binding of cytosolic OMTs to chloroplast envelopes. It is concluded, therefore, that spinach chloroplasts are not involved in the methylation of phenylpropanoid or flavonoid compounds. These results call attention for the re-evaluation of the role of this organelle in the biosynthesis or accumulation of plant secondary metabolites. 
  Reference    Z. Naturforsch. 40c, 331 (1985); received December 11 1984/February 26 1985 
  Published    1985 
  Keywords    Spinacea oleracea, Chenopodiaceae, Chloroplasts, O-Methyltransferase, Caffeic Acid, Quercetin 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0331.pdf 
 Identifier    ZNC-1985-40c-0331 
 Volume    40 
2Author    A. Kio Kobayashi1, JoK. Myong, Im, Kazuyoshi Kawazu0Requires cookie*
 Title    Uptake and Exudation of Phenolic Compounds by Wheat and Antimicrobial Components of the Root Exudate  
 Abstract    In the course of our study, it was found that phenyl propenoic acid derivatives were readily taken up by wheat. Leaf leachate components were chosen for the feeding experiments and /j-coumaric acid, ferulic acid and caffeic acid were found to be quickly taken up into the plants via the roots. The analytical study revealed that the exudate contained potent antim i­ crobial compounds together with amino acids and sugars. Besides the primary metabolites, 4-hydroxystyrene, 3-methoxy-4-hydroxystyrene and 3-methoxy-4-acetoxystyrene were identified as exudate components from wheat roots in sterile hydroponic culture. This indicates that these antimicrobial components may play a significant role in the defense system as allelochemicals for the rhizosphere. 
  Reference    Z. Naturforsch. 51c, 527—533 (1996); received January 31/ 
  Published    1996 
  Keywords    Decarboxylation, p-Coumaric Acid Ferulic Acid, Caffeic Acid, Uptake, Exudate, Antimicrobial Styrenes 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0527.pdf 
 Identifier    ZNC-1996-51c-0527 
 Volume    51