| | 1 | Author
| G. Ünter Schmidt, PeterG. Räber | Requires cookie* | | | Title
| The Rate of ATP Hydrolysis Catalyzed by Reconstituted CF0F i-Liposomes  | | | | Abstract
| The conditions for optimal rates of ATP hydrolysis catalyzed by the chloroplast ATP-synthase (A T P ase), CFoF,, after isolation and reconstitution into asolectin liposomes have been investi gated. The rate of ATP hydrolysis was m easured either after oxidation of CF0F, (by incubation with iodosobenzoate) or after reduction of CFoF, (by incubation with dithiothreitol). In both cases a rate of about 1 -2 A TP (CF0F i-s)" ' was observed under uncoupled conditions. If the pro-teoliposom es are first energized by an acid-base transition and a K"/valinomycin diffusion po ten tial, the uncoupled rate of A TP hydrolysis is about 1 -2 A TP (CFnF, -s) '1 for the oxidized enzyme and about 20 for the reduced species. This rate is about a factor 2 smaller than that observed in chloroolasts under the same conditions. | | | |
Reference
| Z. Naturforsch. 42c, 231 (1987); received O ctober 17 1986 | | | |
Published
| 1987 | | | |
Keywords
| A TP Hydrolysis, ATP Synthase, A TPase, Reconstitution, CF0F, Liposomes | | | |
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| default:Reihe_C/42/ZNC-1987-42c-0231.pdf | | | | Identifier
| ZNC-1987-42c-0231 | | | | Volume
| 42 | |
| 2 | Author
| Petra Fromme, Ingo Dahse, PeterG. Räber | Requires cookie* | | | Title
| Effect of Tentoxin on the Activation and on the Catalytic Reaction of Reconstituted H +-ATPase from Chloroplasts | | | | Abstract
| The proton-translocating ATPase from chloroplasts, C F 0Fj, was isolated, purified and re constituted into asolectin liposomes. The effect o f the energy transfer inhibitor, tentoxin, on different functions o f the enzyme was investigated. Tentoxin does not inhibit the nucleotide release during energization by a pH /A T jum p, i.e. the activation o f the enzyme is not influ enced. ATP synthesis driven by a pH /A T jump and multi-site ATP hydrolysis are completely inhibited by tentoxin, whereas uni-site ATP hydrolysis is not influenced. | | | |
Reference
| Z. Naturforsch. 47c, 239 (1992); received September 30 1991/January 3 1992 | | | |
Published
| 1992 | | | |
Keywords
| H +-ATPase, CF0F, Tentoxin, Enzyme Kinetics, Uni-Site Catalysis | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0239.pdf | | | | Identifier
| ZNC-1992-47c-0239 | | | | Volume
| 47 | |
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