| | 3 | Author
| Dieter Strack | Requires cookie* | | | Title
| Sinapine as a Supply of Choline for the Biosynthesis of Phosphatidylcholine in Raphanus sativus Seedlings  | | | | Abstract
| Biosynthesis o f phosphatidylcholine in young seedlings o f Raphanus sativus is supplied with choline from degradation of the seed constituent sinapine (sinapoylcholine). This conclusion has been deduced from the following results: (1) Raphanus sativus seedlings accumulate approx. 70 nmol phosphatidylcholine which may be relevant for the consumption of choline, liberated during hydrolysis of approx. 130 nmol sinapine. (2) [14C]choline and [14C]ethanolamine, taken up by germinating Raphanus seeds, are exclu sively consumed in the biosynthesis o f phosphatidylcholine and phosphatidylethanolamine, respectively. (3) Pulse-chase experiments with seedlings at different germination stages show a reduced [14C]choline incorporation into phosphatidylcholine at the time when degradation o f sinapine occurs, obviously as a result of an isotope dilution by an increase o f the endogenous choline pool. (4) After [14C]choline pulse to immature seeds, during the process o f seed maturation most of the activity taken up is incorporated into accumulating sinapine and approx. 50% compared to this into phosphatidylcholine. During seedling development the quantity o f labelled sinapine rapidly decreases as a result of sinapine degradation with a concomitant label increase in free choline, phosphorylcholine, and phosphatidylcholine. Approx. 50% of the choline liberated from sinapine, is consumed in the biosynthesis of phosphatidylcholine. High performance liquid chromatographic analyses of phosphatidylcholine during Raphanus germination revealed that this phospholipid might be a metabolically active compound. Changes in the absorptivity of this compound at 210 nm indicate changes in the degree of unsaturation in the fatty acyl groups. | | | |
Reference
| Z. Naturforsch. 36c, 215—221 (1981); received December 231980 | | | |
Published
| 1981 | | | |
Keywords
| Sinapine, Phosphatidylcholine, Phenylpropanoids, Phospholipids, Raphanus, Brassicaceae | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0215.pdf | | | | Identifier
| ZNC-1981-36c-0215 | | | | Volume
| 36 | |
| 4 | Author
| V. Hemleben | Requires cookie* | | | Title
| Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br | | | | Abstract
| Several lines of the genetically well defined Matthiola incana (Brassicaceae) concerning the anthocyanin biosynthesis (line 02, 06, 10, and 14) store the anthocyanin pigment as crystals (line 06) or in anthocyanoblasts ("balls") in the epidermal cells (line 02, 10, and 14) o f the flower petals. The genetic constitution o f these genotypes is in addition to the basic factors f +, g+, and e+, b+b+ or bb (cyanidin or pelargonidin type), 11, u+u+, and v+v+ or vv, demonstrating that the 11, u+u+ constitution is responsible for precipitation of anthocyanins in a certain structure indepen dent of the other modificationally acting genes b and v. The 11, u+u+ types accumulate preferentially acylated 3-biosides. | | | |
Reference
| Z. Naturforsch. 36c, 925 (1981); received September 29 1981 | | | |
Published
| 1981 | | | |
Keywords
| Anthocyanin, 3-Biosides, Crystals, Matthiola incana, Brassicaceae | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0925.pdf | | | | Identifier
| ZNC-1981-36c-0925 | | | | Volume
| 36 | |
| 6 | Author
| M. Ichael Linscheid, Zentralbereich Forschung, Entwicklung Der, Bayer Ag, D. Leverkusen, Ieter Strack | Requires cookie* | | | Title
| The Structures of Sinapic Acid Esters and Their Metabolism in Cotyledons of Raphanus sativus | | | | Abstract
| Five sinapic acid esters were isolated from cotyledons of Raphanus sativus and have been iden tified with the aid of electron impact (El)-, field desorption (FD)-mass spectrometry and NMR spectroscopy: 6,3'-disinapoylsucrose, sinapoylcholine (sinapine), 6 -sinapoylglucoraphenine, 1 -si napoylglucose, and sinapoylmalate. Three of these derivatives are metabolically related in the se quence sinapoylcholine -» • 1 -sinapoylglucose — ► sinapoylmalate. | | | |
Reference
| Z. Naturforsch. 35c, 907—914 (1980); received August 5 1980 | | | |
Published
| 1980 | | | |
Keywords
| Raphanus sativus, Brassicaceae, Sinapic Acid Esters, Phenylpropanoids, High Performance Liquid Chromatography | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0907.pdf | | | | Identifier
| ZNC-1980-35c-0907 | | | | Volume
| 35 | |
| 7 | Author
| G. Erhild, N. Urm, Ann, D. Ieter Strack | Requires cookie* | | | Title
| Sinapine Esterase I. Characterization of Sinapine Esterase from Cotyledons of Raphanus sativus | | | | Abstract
| From cotyledons o f Raphanus sativus (red radish) an esterase activity which catalyzes the hy drolysis o f sinapine into sinapic acid and choline has been isolated. The enzyme, which has a near absolute specificity, is not analogous with any esterase described in the literature. The reaction has a pH optim um o f 8.5 and the apparent K m is 1.95 x 10~5 m. The enzyme is relatively insensi tive to both physostigm ine (eserine) {K x = 1.73 x 10-4 m) and neostigm ine (A'i = 2 .1 3 x 10-4 m). Diisopropyl fluorophosphate (D F P) showed no inhibition and diethyl /?-nitrophenylphosphate (E 600) only a slight inhibitory effect at 10-5 m, respectively. Choline (10~2 m) was inhibitory but acetylcholine (1 0 -2 m) stimulated the enzyme activity. | | | |
Reference
| Z. Naturforsch. 34c, 715—720 (1979); received June 11 1979 | | | |
Published
| 1979 | | | |
Keywords
| Esterase, Sinapine, Sinapic Acid Esters, Raphanus, Brassicaceae, High-Performance Liquid Chro matography (HPLC) | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0715.pdf | | | | Identifier
| ZNC-1979-34c-0715 | | | | Volume
| 34 | |
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