ZfN: Search Results

1	Author	Dieter Strack	Requires cookie*
	Title	Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings
	Abstract	Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin ce the addition of UDP results in a breakdown o f the ester.
	Reference	Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979
	Published	1980
	Keywords	Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0204.pdf
	Identifier	ZNC-1980-35c-0204
	Volume	35

2	Author	Norbert Tkotz, D. Ieter Strack	Requires cookie*
	Title	from Raphanus sativus C otyledons
	Abstract	Protein preparations from cotyledons of Raphanus sati vus transfer the sinapoyl moiety of 1 -sinapoylglucose to L-malate to form sinapoyl-L-malate. To our knowledge this is the first time that this type of reaction has been found to be involved in phenylpropanoid depside formation. Most acylation reactions have so far been demonstrated to depend on acyl-CoAs and only one example is known describing 1-O-acyl sugar derivatives as acyl donors.
	Reference	Z. Naturforsch. 35c, 835—837 (1980); received June 261980
	Published	1980
	Keywords	Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Transacylation, Sinapoyltransferase
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0835_n.pdf
	Identifier	ZNC-1980-35c-0835_n
	Volume	35

3	Author	M. Ichael Linscheid, Zentralbereich Forschung, Entwicklung Der, Bayer Ag, D. Leverkusen, Ieter Strack	Requires cookie*
	Title	The Structures of Sinapic Acid Esters and Their Metabolism in Cotyledons of Raphanus sativus
	Abstract	Five sinapic acid esters were isolated from cotyledons of Raphanus sativus and have been iden tified with the aid of electron impact (El)-, field desorption (FD)-mass spectrometry and NMR spectroscopy: 6,3'-disinapoylsucrose, sinapoylcholine (sinapine), 6 -sinapoylglucoraphenine, 1 -si napoylglucose, and sinapoylmalate. Three of these derivatives are metabolically related in the se quence sinapoylcholine -» • 1 -sinapoylglucose — ► sinapoylmalate.
	Reference	Z. Naturforsch. 35c, 907—914 (1980); received August 5 1980
	Published	1980
	Keywords	Raphanus sativus, Brassicaceae, Sinapic Acid Esters, Phenylpropanoids, High Performance Liquid Chromatography
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0907.pdf
	Identifier	ZNC-1980-35c-0907
	Volume	35