| | 1 | Author
| Peter Hanfland, Gerd Assmann, Heinz Egge | Requires cookie* | | | Title
| Enzymatic Determination of the Anomeric Structures of Two Blood-Group B Active Glycosphingolipids Recombined with Apolipoproteins  | | | | Abstract
| Anomeric configuration of oligosaccharides usually is established by specific glycosidases. For this purpose detergents achieving w ater solubility of prim arily insoluble glycosphingolipids as sub strates have been replaced by delipidated hum an serum high density lipoproteins. The new method, tested by several well characterized glycosphingolipids and glycosidases, finally was applied to the evaluation of anomeric structures of two blood-group B active glycosphingolipids [ceramide hexa-saccharide (B-I) and ceramide octasaccharide (B -II)] from hum an erythrocyte membranes. In both B-I and B-II, a-glycosidic linkage was dem onstrated for the term inal galactose and fucose residues. /?-glycosidic linkage has been evaluated for backbone saccharides. Together with the results pre viously obtained by composition analysis, linkage analysis and sequence analysis the following complete structure can be established: B -I: G a lal -> 3Gal (2 l<xFuc) ß \ -> 4G lcN A c^l -> 3Gal/?l -> 4Glc/?l -> I C e r; B-II: Galcd -> 3G al(2 lcvF uc)^ l -> 4GlcNAc/>T 3Gal/?l -> 4G lcN A c^l 3Galy5l -> 4Glc/?l — ICer. | | | |
Reference
| Z. Naturforsch. 33c, 73 (1978); received July 14/October 3 1977 | | | |
Published
| 1978 | | | |
Keywords
| Blood-Group Glycolipids, Anomeric Structure, Enzym atic Degradation, Apolipoproteins | | | |
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| default:Reihe_C/33/ZNC-1978-33c-0073.pdf | | | | Identifier
| ZNC-1978-33c-0073 | | | | Volume
| 33 | |
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